Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Ammonium Sulphate Precipitation
ab71003 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/10 - 1/50. Detects a band of approximately 65 kDa (predicted molecular weight: 71 kDa).
Anti-PARP6 antibody - C-terminal 图像
Western blot - PARP6 antibody - C-terminal (ab71003)
All lanes :
Anti-PARP6 antibody - C-terminal (ab71003) at 1/10 dilutionLane 1 :
Nontransfected 293 cell lysates Lane 2 :
293 cell lysates transiently transfected with the PARP6 gene
Lysates/proteins at 2 µg per lane.Predicted band size :
71 kDaObserved band size :
65 kDa (why is the actual band size different from the predicted?
)Additional bands at :
25 kDa,40 kDa,57 kDa,72 kDa,95 kDa. We are unsure as to the identity of these extra bands.
Anti-PARP6 antibody - C-terminal (ab71003)参考文献
has not yet been referenced specifically in any publications.
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