1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
相关性Component of the vault ribonucleoprotein particle which is at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1.
Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.
细胞定位Cytoplasmic, but is also found in the nucleus, associated with mitotic spindles.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling PARP4 with ab86066 at 1/200 (1µg/ml). Detection: DAB.
Western blot - PARP4 antibody (ab86066)
All lanes : Anti-PARP4 antibody (ab86066) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg Lane 2 : Hela whole cell lysate at 15 µg Lane 3 : Hela whole cell lysate at 5 µg
developed using the ECL technique
Predicted band size : 193 kDa
Exposure time : 3 minutes
Immunoprecipitation - PARP4 antibody (ab86066)
1 mg whole cell lysate from HeLa cells was immunoprecipitated using 3 µg of ab86066. 20% of IP was loaded in each lane and probed with ab86066 at 0.4 µg/ml (lane 1) or with a control IgG (lane 2). Detection: chemiluminescence with an exposure time of 30 seconds.
Anti-PARP4 antibody (ab86066)参考文献
has not yet been referenced specifically in any publications.