Anti-PARP3抗体(ab96601)

概述

  • 产品名称Anti-PARP3抗体
    参阅全部 PARP3 一抗
  • 描述
    兔多克隆抗体to PARP3
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 63-340 of Human PARP3 (NP_005476).

  • 阳性对照
    • A431, HeLa S3, 293T, H1299, HepG2, MOLT4 and Raji cell lysates for WB. OVCA tissue for IHC-P.

性能

应用

Our Abpromise guarantee covers the use of ab96601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/250.

靶标

  • 功能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. Binds DNA. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing.
  • 组织特异性Widely expressed; the highest levels are in the kidney, skeletal muscle, liver, heart and spleen; also detected in pancreas, lung, placenta, brain, leukocytes, colon, small intestine, ovary, testis, prostate and thymus.
  • 序列相似性Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
  • 结构域According to PubMed:10329013, the N-terminal domain (54 amino acids) of isoform 2 is responsible for its centrosomal localization. The C-terminal region contains the catalytic domain.
  • 翻译后修饰Auto-poly(ADP)-ribosylation.
  • 细胞定位Nucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Core component of the centrosome. Preferentially localized to the daughter centriole throughout the cell cycle. According to PubMed:16924674, it is almost exclusively localized in the nucleus and appears in numerous small foci and a small number of larger foci whereas a centrosomal location has not been detected.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADP ribosyltransferase (NAD+; poly (ADP ribose) antibody
    • ADP ribosyltransferase diphtheria toxin like 3 antibody
    • ADPRT-3 antibody
    • ADPRT3 antibody
    • ADPRTL2 antibody
    • ADPRTL3 antibody
    • ARTD3 antibody
    • hPARP 3 antibody
    • hPARP-3 antibody
    • hPARP3 antibody
    • IRT 1 antibody
    • IRT1 antibody
    • NAD(+) ADP ribosyltransferase 3 antibody
    • NAD(+) ADP-ribosyltransferase 3 antibody
    • NAD+ ADP ribosyltransferase 3 antibody
    • pADPRT 3 antibody
    • pADPRT-3 antibody
    • pADPRT3 antibody
    • PARP 3 antibody
    • PARP-3 antibody
    • PARP3 antibody
    • PARP3_HUMAN antibody
    • Poly (ADP ribose) polymerase family, member 3 antibody
    • Poly (ADP ribose) synthetase 3 antibody
    • Poly [ADP-ribose] polymerase 3 antibody
    • Poly[ADP ribose] synthetase 3 antibody
    • Poly[ADP-ribose] synthase 3 antibody
    see all

Anti-PARP3 antibody 图像

  • ab96601, at 1/100 dilution staining PARP3 in paraffin-embedded OVCA by Immunohistochemistry.
  • All lanes : Anti-PARP3 antibody (ab96601) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa S3 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 60 kDa

Anti-PARP3 antibody (ab96601)参考文献

ab96601 has not yet been referenced specifically in any publications.

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Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 40 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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提交于 May 31 2012

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 40 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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提交于 May 29 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"