Anti-PARP2抗体(ab115620)

概述

  • 产品名称Anti-PARP2抗体
    参阅全部 PARP2 一抗
  • 描述
    山羊多克隆抗体to PARP2
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    LDLFEVEKDGEKE

    with a Cysteine residue linker, corresponding to internal sequence amino acids 402-414 of Human PARP2 (NP_005475.2).

  • 阳性对照
    • Human Adrenal andSkin tissues; Human Spleen lysate.

性能

应用

Our Abpromise guarantee covers the use of ab115620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 66 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/8000.

靶标

  • 功能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
  • 组织特异性Widely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus.
  • 序列相似性Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
  • 翻译后修饰Poly-ADP-ribosylated by PARP1.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADP ribosyltransferase like 2 antibody
    • ADP-ribosyltransferase diphtheria toxin-like 2 antibody
    • ADPRT 2 antibody
    • ADPRT-2 antibody
    • ADPRT2 antibody
    • ADPRTL 2 antibody
    • ADPRTL 3 antibody
    • ADPRTL2 antibody
    • ADPRTL3 antibody
    • ARTD2 antibody
    • hPARP 2 antibody
    • hPARP-2 antibody
    • hPARP2 antibody
    • NAD(+) ADP ribosyltransferase 2 antibody
    • NAD(+) ADP-ribosyltransferase 2 antibody
    • pADPRT 2 antibody
    • pADPRT-2 antibody
    • pADPRT2 antibody
    • PARP 2 antibody
    • PARP-2 antibody
    • PARP2 antibody
    • PARP2_HUMAN antibody
    • Poly (ADP ribose) polymerase family member 2 antibody
    • Poly (ADP ribosyl) transferase like 2 antibody
    • poly (ADP-ribose) polymerase 2 antibody
    • Poly [ADP ribose] synthetase 2 antibody
    • Poly [ADP-ribose] polymerase 2 antibody
    • Poly(ADP ribose) synthetase antibody
    • Poly[ADP-ribose] synthase 2 antibody
    see all

Anti-PARP2 antibody 图像

  • ab115620, at 2.5µg/ml, staining PARP2 in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115620, at 2.5µg/ml, staining PARP2 in Formalin-fixed, Paraffin-embedded Human Skin tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-PARP2 antibody (ab115620) at 1 µg/ml + Human Spleen Lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 66 kDa

Anti-PARP2 antibody (ab115620)参考文献

ab115620 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% TritonX100 in PBS
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Aug 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"