概述

  • 产品名称Anti-PARP16抗体
    参阅全部 PARP16 一抗
  • 描述
    兔多克隆抗体to PARP16
  • 经测试应用适用于: WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 252-301 (PKYFVVTNNQ LLRVKYLLVY SQKPPKSRAS SQLSWFSSHW FTVMISLYLL) of Human PARP16 (NP_060321).

  • 阳性对照
    • 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration. PubMed: 22701565
IP Use at an assay dependent concentration. PubMed: 22701565

靶标

Anti-PARP16 antibody 图像

  • Anti-PARP16 antibody (ab84641) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
  • Immunofluorescence analysis of HeLa cells, staining endogenous PARP16 using ab84641, at 1/50 dilution.
  • Immunofluorescence analysis of HeLa cells, staining endogenous PARP16 using ab84641, at 1/50 dilution.

Anti-PARP16 antibody (ab84641)参考文献

This product has been referenced in:
  • Di Paola S  et al. PARP16/ARTD15 Is a Novel Endoplasmic-Reticulum-Associated Mono-ADP-Ribosyltransferase That Interacts with, and Modifies Karyopherin-ß1. PLoS One 7:e37352 (2012). WB, ICC/IF, IP ; Human . Read more (PubMed: 22701565) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab84641.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"