The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Poly(ADP-ribosyl)ation is a DNA-damage-dependent post-translational modification of histones. Poly(ADP-ribose) polymerases (PARPs) are a family of 18 proteins, encoded by different genes and displaying a conserved catalytic domain.