Anti-PARP1抗体(ab75757)

概述

  • 产品名称
  • 描述
    鸡多克隆抗体to PARP1
  • 特异性
    In WB, ab75757 recognizes intact PARP1 (113kDa), the 89kDa apoptosis-induced cleavage product, and other lower molecular weight fragments of PARP1.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein corresponding to Human PARP1. Highly purified human PARP1 expressed in baculovirus.

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab75757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 113 kDa.

Use at a concentration of 1 µg/ml (HRP/TMB colorimetric) or <1 µg/ml (ECL). 

ELISA Use at an assay dependent concentration.

靶标

  • 功能
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
  • 序列相似性
    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • 翻译后修饰
    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
    • ADP ribosyltransferase antibody
    • ADP ribosyltransferase diphtheria toxin like 1 antibody
    • ADP ribosyltransferase NAD(+) antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • ARTD1 antibody
    • msPARP antibody
    • NAD(+) ADP ribosyltransferase 1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • pADPRT 1 antibody
    • pADPRT1 antibody
    • PARP 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly (ADP ribose) polymerase 1 antibody
    • poly (ADP ribose) polymerase family, member 1 antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly(ADP ribose) polymerase antibody
    • poly(ADP ribose) synthetase antibody
    • poly(ADP ribosyl)transferase antibody
    • Poly[ADP ribose] synthetase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    see all

Anti-PARP1 antibody 图像

  • All lanes : Anti-PARP1 antibody (ab75757) at 1/1000 dilution

    Lane 1 : Extract of HeLa cells treated with vehicle
    Lane 2 : Extract of HeLa cells treated with staurosporine
    Lane 3 : Extract of Jurkat cells treated with vehicle
    Lane 4 : Extract of Jurkat cells treated with staurosporine
    Lane 5 : Extract of C6 cells treated with vehicle
    Lane 6 : Extract of C6 cells treated with camptothecin
    Lane 7 : Extract of NIH 3T3 cells treated with vehicle
    Lane 8 : Extract of NIH 3T3 cells treated with staurosporine

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-chicken IgY at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 113 kDa


    Exposure time : 1 minute

    SDS PAGE performed under reducing conditions (100mM DTT, Sample heated at 50°C).

    Blocking: in 5% Milk + PBS for 3 hours at RT.
    Primary antibody diluted in 5% Milk + PBS and incubated overnight at 4°C.
    Secondary antibody diluted in 5% Milk + PBS and incubated for 2 hour at RT.
    Predicted band size : 113 kDa: 89 kDa and 24 kDa.
    Observed band size : 113 kDa: 89 kDa and 24 kDa.

  • All lanes : Anti-PARP1 antibody (ab75757) at 1 µg/ml

    Lane 1 : Jurkat cell lysate at 5 µg
    Lane 2 : Apoptotic Jurkat cell lysate, induced with camptothecin for 4 hr

    Secondary
    GAC-HRP at 1/2000 dilution

    Predicted band size : 113 kDa
    Additional bands at : 89 kDa (possible cleavage fragment).

    Note other smaller apoptosis-induced PARP1 fragments in Lane 2.

实验方案

Anti-PARP1 antibody (ab75757)参考文献

ab75757 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. We have several antibodies which can be used to detect PARP in Western blotting. From the study I believe you wish to detect both the cleaved (C-terminal fragment) and the full length PARP form in mouse samples? Thi...

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