概述

  • 产品名称
  • 描述
    兔多克隆抗体to PARP1
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Cow PARP1 (C terminal).

  • 阳性对照
    • WB: Raji cell lysate. IF/ICC: Hek293 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab75607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 113 kDa.

靶标

  • 功能
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
  • 序列相似性
    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • 翻译后修饰
    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
    • ADP ribosyltransferase antibody
    • ADP ribosyltransferase diphtheria toxin like 1 antibody
    • ADP ribosyltransferase NAD(+) antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • ARTD1 antibody
    • msPARP antibody
    • NAD(+) ADP ribosyltransferase 1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • pADPRT 1 antibody
    • pADPRT1 antibody
    • PARP 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly (ADP ribose) polymerase 1 antibody
    • poly (ADP ribose) polymerase family, member 1 antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly(ADP ribose) polymerase antibody
    • poly(ADP ribose) synthetase antibody
    • poly(ADP ribosyl)transferase antibody
    • Poly[ADP ribose] synthetase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    see all

Anti-PARP1 antibody 图像



  • Predicted band size : 113 kDa

    Lane 1: HeLa cell extract treated with vehicle (20 µg).
    Lane 2: HeLa cells extract treated with staurosporine (20 µg).
    Lane 3: Jurkat cell extract treated with vehicle (20 µg).
    Lane 4: Jurkat cell extract treated with staurosporine (20 µg).

    SDS PAGE performed under reducing conditions (100mM DTT, sample heated at 50°C).

    Primary (Lanes 1-4): ab75607 at 2 µg/mL in 5% milk + PBS overnight at 4°C.
    Secondary (Lanes 1-4): HRP-conjugated goat anti-rabbit IgG (H&L) at 1/10000 in 5% milk + PBS for 2 hour at room temperature.

    Developed: ECL with 30 second exposure. Blocked in 5% milk + PBS for 3 hours at room temperature.

    Predicted band size: 113 kDa and 89 kDa.
    Observed band size: 113 kDa and 89 kDa.

  • ICC/IF image of ab75607 stained HEK293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab756007, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-PARP1 antibody (ab75607) at 10 µg/ml + Raji cell lysate

    Predicted band size : 113 kDa
    Observed band size : 113 kDa

Anti-PARP1 antibody (ab75607)参考文献

This product has been referenced in:
  • Chen Y  et al. Hydroquinone-induced malignant transformation of TK6 cells by facilitating SIRT1-mediated p53 degradation and up-regulating KRAS. Toxicol Lett 259:133-42 (2016). WB, IHC-P ; Human . Read more (PubMed: 27515134) »

See 1 Publication for this product

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