Anti-PARP1抗体(ab194586)
Key features and details
- Rabbit polyclonal to PARP1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-PARP1抗体
参阅全部 PARP1 一抗 -
描述
兔多克隆抗体to PARP1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PARP1 aa 150-250. The exact sequence is proprietary.
Database link: P09874 -
阳性对照
- WB: Extracts from A549, Jurkat, HeLa, K562, Raji, HepG2 and Y79 cell lines. Mouse testis lysate. ICC/IF: NIH/3T3 and U-2 OS cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab194586于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/20 - 1/50.
|
|
WB | (1) |
1/1000 - 1/2000. Predicted molecular weight: 113 kDa.
|
ICC/IF |
1/50 - 1/100.
|
说明 |
---|
IHC-P
1/20 - 1/50. |
WB
1/1000 - 1/2000. Predicted molecular weight: 113 kDa. |
ICC/IF
1/50 - 1/100. |
靶标
-
功能
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. -
序列相似性
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
翻译后修饰
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 142 Human
- Entrez Gene: 11545 Mouse
- Entrez Gene: 25591 Rat
- Omim: 173870 Human
- SwissProt: P09874 Human
- SwissProt: P11103 Mouse
- SwissProt: P27008 Rat
- Unigene: 177766 Human
see all -
别名
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
- ADP ribosyltransferase antibody
- ADP ribosyltransferase diphtheria toxin like 1 antibody
see all
图片
-
All lanes : Anti-PARP1 antibody (ab194586) at 1/1000 dilution
Lane 1 : Extract from A549 (Human lung carcinoma cell line) cell line
Lane 2 : Extract from Jurkat (Human T cell leukemia cell line from peripheral blood) cell line
Lane 3 : Extract from Hela (Human epithelial cell line from cervix adenocarcinoma) cell line
Lane 4 : Extract from K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell line
Lane 5 : Extract from Raji (Human Burkitt's lymphoma cell line) cell line
Lane 6 : Extract from HepG2 (Human liver hepatocellular carcinoma cell line) cell line
Lane 7 : Extract from Y79 (Human retinoblastoma cell line) cell line
Predicted band size: 113 kDa -
U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for PARP1 using ab194586 at a dilution of 1/100 in ICC/IF. Blue: DAPI for nuclear staining.
-
Immunohistochemistry of Human breast cancer tissue staining PARP1 with ab194586
-
Immunohistochemistry of Human appendix tissue staining PARP1 with ab194586
-
Immunohistochemistry of rat testis tissue staining PARP1 with ab194586
-
NIH-3T3 (Mouse embryo fibroblast cell line) cells stained for PARP1 using ab194586 at a dilution of 1/100 in ICC/IF. Blue: DAPI for nuclear staining.
-
Anti-PARP1 antibody (ab194586) at 1/1000 dilution + Mouse testis lysate at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
Exposure time: 90 secondsBlocking buffer: 3% non-fat dry milk in TBST.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (15)
ab194586 被引用在 15 文献中.
- Zhang L et al. Positive Feedback Regulation of Poly(ADP-ribose) Polymerase 1 and the DNA-PK Catalytic Subunit Affects the Sensitivity of Nasopharyngeal Carcinoma to Etoposide. ACS Omega 7:2571-2582 (2022). PubMed: 35097256
- Zhang H et al. [18F]-Labeled PARP-1 PET imaging of PSMA targeted alpha particle radiotherapy response. Sci Rep 12:13034 (2022). PubMed: 35906379
- Biswas S et al. PEITC: A resounding molecule averts metastasis in breast cancer cells in vitro by regulating PKCδ/Aurora A interplay. Heliyon 8:e11656 (2022). PubMed: 36458309
- D'Onofrio N et al. Colorectal Cancer Apoptosis Induced by Dietary δ-Valerobetaine Involves PINK1/Parkin Dependent-Mitophagy and SIRT3. Int J Mol Sci 22:N/A (2021). PubMed: 34360883
- Xu L et al. Quantitative global proteome and phosphorylome analyses reveal potential biomarkers in kidney cancer. Oncol Rep 46:N/A (2021). PubMed: 34528699