Anti-PARP抗体(ab75757)

概述

  • 产品名称Anti-PARP抗体
    参阅全部 PARP 一抗
  • 描述
    鸡多克隆抗体to PARP
  • 特异性In WB, ab75757 recognizes intact PARP (113kDa), the 89kDa apoptosis-induced cleavage product, and other lower molecular weight fragments of PARP.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length highly purified human PARP expressed in baculovirus.

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab75757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 µg/ml (HRP/TMB colorimetric) or <1 µg/ml (ECL). Predicted molecular weight: 113 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
    • 序列相似性Contains 1 BRCT domain.
      Contains 1 PARP alpha-helical domain.
      Contains 1 PARP catalytic domain.
      Contains 2 PARP-type zinc fingers.
    • 翻译后修饰Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
      Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
      S-nitrosylated, leading to inhibit transcription regulation activity.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
      • ADP ribosyltransferase diphtheria toxin like 1 antibody
      • ADP ribosyltransferase NAD(+) antibody
      • ADPRT 1 antibody
      • ADPRT antibody
      • ADPRT1 antibody
      • ARTD1 antibody
      • msPARP antibody
      • NAD(+) ADP ribosyltransferase 1 antibody
      • NAD(+) ADP-ribosyltransferase 1 antibody
      • pADPRT 1 antibody
      • pADPRT1 antibody
      • PARP 1 antibody
      • PARP antibody
      • PARP-1 antibody
      • PARP1 antibody
      • PARP1_HUMAN antibody
      • Poly (ADP ribose) polymerase 1 antibody
      • poly (ADP ribose) polymerase family, member 1 antibody
      • Poly [ADP-ribose] polymerase 1 antibody
      • Poly(ADP ribose) polymerase antibody
      • poly(ADP ribose) synthetase antibody
      • poly(ADP ribosyl)transferase antibody
      • Poly[ADP ribose] synthetase 1 antibody
      • Poly[ADP-ribose] synthase 1 antibody
      • PPOL antibody
      see all

    Anti-PARP antibody 图像



    • Predicted band size : 113 kDa
      Lane 1 = Extract of HeLa cells treated with vehicle ? 20 ug. Lane 2 = Extract of HeLa cells treated with staurosporine ? 20 ug. Lane 3 = Extract of Jurkat cells treated with vehicle ? 20 ug. Lane 4 = Extract of Jurkat cells treated with staurosporine ? 20 ug. Lane 5 = Extract of C6 cells treated with vehicle ? 20 ug. Lane 6 = Extract of C6 cells treated with camptothecin ? 20 ug. Lane 7 = Extract of NIH 3T3 cells treated with vehicle ? 20 ug. Lane 8 = Extract of NIH 3T3 cells treated with staurosporine ? 20 ug. SDS PAGE performed under reducing conditions (100mM DTT ? Sample heated at 50?C). Primary : Lanes 1-8: Anti PARP antibody (ab75757) at 1:1000 dilution. Secondary : Lanes 1-8: Goat anti chicken IgY-HRP at 1:10000. Developed: ECL with 1 min exposure. Blocking: in 5% Milk + PBS for 3 hours at RT. Primary antibody: in 5% Milk + PBS overnight at 4 C. Secondary antibody: in 5% Milk + PBS for 2 hour at RT. Predicted band size : 113 kDa: 89 kDa and 24 kDa. Observed band size : 113 kDa: 89 kDa and 24 kDa.
    • All lanes : Anti-PARP antibody (ab75757) at 1 µg/ml

      Lane 1 : Jurkat cell lysate at 5 µg
      Lane 2 : Apoptotic Jurkat cell lysate, induced with camptothecin for 4 hr

      Secondary
      GAC-HRP at 1/2000 dilution

      Predicted band size : 113 kDa
      Additional bands at : 89 kDa (possible cleavage fragment).
      Note other smaller apoptosis-induced PARP fragments in Lane 2.

    Anti-PARP antibody (ab75757)参考文献

    ab75757 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. We have several antibodies which can be used to detect PARP in Western blotting. From the study I believe you wish to detect both the cleaved (C-terminal fragment) and the full length PARP form in mouse samples? Thi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"