The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 113 kDa.
功能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
翻译后修饰Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. S-nitrosylated, leading to inhibit transcription regulation activity.
ADP ribosyltransferase diphtheria toxin like 1 antibody
ADP ribosyltransferase NAD(+) antibody
ADPRT 1 antibody
NAD(+) ADP ribosyltransferase 1 antibody
NAD(+) ADP-ribosyltransferase 1 antibody
pADPRT 1 antibody
PARP 1 antibody
Poly (ADP ribose) polymerase 1 antibody
poly (ADP ribose) polymerase family, member 1 antibody
Poly [ADP-ribose] polymerase 1 antibody
Poly(ADP ribose) polymerase antibody
poly(ADP ribose) synthetase antibody
poly(ADP ribosyl)transferase antibody
Poly[ADP ribose] synthetase 1 antibody
Poly[ADP-ribose] synthase 1 antibody
Anti-PARP antibody 图像
Western blot - Anti-PARP antibody (ab75607)
Predicted band size : 113 kDa Lane 1 = Extract of HeLa cells treated with vehicle ? 20 ug. Lane 2 = Extract of HeLa cells treated with staurosporine ? 20 ug. Lane 3 = Extract of Jurkat cells treated with vehicle ? 20 ug. Lane 4 = Extract of Jurkat cells treated with staurosporine ? 20 ug. SDS PAGE performed under reducing conditions (100mM DTT ? Sample heated at 50?C). Primary : Lanes 1-4: Anti PARP antibody (ab75607) at 2 ug/mL. Secondary : Lanes 1-4: Goat anti rabbit IgG(H&L)-HRP at 1:10000. Developed: ECL with 30 sec exposure. Blocking: in 5% Milk + PBS for 3 hours at RT. Primary antibody: in 5% Milk + PBS overnight at 4 C. Secondary antibody: in 5% Milk + PBS for 2 hour at RT. Predicted band size : 113 kDa and 89 kDa. Observed band size : 113 kDa and 89 kDa
ICC/IF image of ab75607 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab756007, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - PARP antibody (ab75607)
Anti-PARP antibody (ab75607) at 10 µg/ml + Raji cell lysate
Predicted band size : 113 kDa Observed band size : 113 kDa
Anti-PARP antibody (ab75607)参考文献
This product has been referenced in:
Chen Y et al. Hydroquinone-induced malignant transformation of TK6 cells by facilitating SIRT1-mediated p53 degradation and up-regulating KRAS. Toxicol Lett259:133-42 (2016).
Read more (PubMed: 27515134) »