概述

  • 产品名称Anti-PARK7/DJ1抗体
    参阅全部 PARK7/DJ1 一抗
  • 描述
    山羊多克隆抗体to PARK7/DJ1
  • 经测试应用适用于: IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human PARK7/DJ1 aa 178-189 (C terminal).
    Sequence:

    C-AAQVKAPLVLKD


    (Peptide available as ab22993)

  • 阳性对照
    • WB: Jurkat, HeLa and 3T3 whole cell lysate. Human, fetal and adult mouse and rat brain lysates.

性能

应用

Our Abpromise guarantee covers the use of ab4150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human PARK7/DJ1 peptide (ab22993).

靶标

  • 功能Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone.
  • 组织特异性Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain. Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.
  • 疾病相关Defects in PARK7 are the cause of Parkinson disease type 7 (PARK7) [MIM:606324]. A neurodegenerative disorder characterized by resting tremor, postural tremor, bradykinesia, muscular rigidity, anxiety and psychotic episodes. PARK7 has onset before 40 years, slow progression and initial good response to levodopa. Some patients may show traits reminiscent of amyotrophic lateral sclerosis-parkinsonism/dementia complex (Guam disease).
  • 序列相似性Belongs to the peptidase C56 family.
  • 翻译后修饰Sumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity.
    Cys-106 is easily oxidized to sulfinic acid.
    Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.
  • 细胞定位Cytoplasm. Nucleus. Mitochondrion. Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAP1 antibody
    • DJ-1 antibody
    • DJ1 antibody
    • DJ1 protein antibody
    • Epididymis secretory sperm binding protein Li 67p antibody
    • FLJ27376 antibody
    • FLJ34360 antibody
    • FLJ92274 antibody
    • HEL S 67p antibody
    • Oncogene DJ1 antibody
    • OTTHUMP00000001348 antibody
    • OTTHUMP00000001349 antibody
    • OTTHUMP00000001350 antibody
    • OTTHUMP00000001351 antibody
    • PARK7 antibody
    • PARK7_HUMAN antibody
    • Parkinson disease (autosomal recessive, early onset) 7 antibody
    • Parkinson disease protein 7 antibody
    • Parkinson protein 7 antibody
    • Protein DJ-1 antibody
    • SP22 antibody
    see all

Anti-PARK7/DJ1 antibody 图像

  • All lanes : Anti-PARK7/DJ1 antibody (ab4150) at 0.001 µg/ml

    Lane 1 : HeLa cell lysate (in RIPA buffer)
    Lane 2 : Jurkat cell lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 20 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

  • All lanes : Anti-PARK7/DJ1 antibody (ab4150) at 0.001 µg/ml

    Lane 1 : Human cerebellum lysate (in RIPA buffer)
    Lane 2 : Human frontal cortex lysate (in RIPA buffer)
    Lane 3 : Human hippocampus lysate (in RIPA buffer)
    Lane 4 : Mouse fetal brain lysate (in RIPA buffer)
    Lane 5 : Mouse brain lysate (in RIPA buffer)
    Lane 6 : Rat brain lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 20 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.



  • Predicted band size : 20 kDa

    Western blot using ab4150 at 1/500.
    Exposure time: 30s.

    Left hand lane: HeLa whole cell lysate (human)
    Right hand lane: 3T3 whole cell lysate (mouse)

  • Anti-PARK7/DJ1 antibody (ab4150) at 1/6000 dilution + Mouse brain tissue lysate - whole at 5 µg

    Secondary
    HRP-conjugated Donkey anti-mouse IgG at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-PARK7/DJ1 antibody (ab4150)参考文献

This product has been referenced in:
  • Johnson WM  et al. Regulation of DJ-1 by Glutaredoxin 1 in Vivo: Implications for Parkinson's Disease. Biochemistry 55:4519-32 (2016). WB ; Mouse . Read more (PubMed: 26894491) »
  • Moscovitz O  et al. The Parkinson's-associated protein DJ-1 regulates the 20S proteasome. Nat Commun 6:6609 (2015). WB . Read more (PubMed: 25833141) »

See all 12 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Medaka fish Tissue lysate - whole (whole brain)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 5 µg
Specification whole brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Tyrone Genade

Verified customer

提交于 Sep 21 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Nothobranchius furzeri Tissue lysate - whole (whole brain)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 5 µg
Specification whole brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Tyrone Genade

Verified customer

提交于 Sep 21 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (whole brain)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 5 µg
Specification whole brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Tyrone Genade

Verified customer

提交于 Sep 21 2016

Application Western blot
Sample Rat Tissue lysate - whole (midbrain)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 10 µg
Specification midbrain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Tyrone Genade

Verified customer

提交于 Sep 06 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Sep 16 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Tissue lysate - whole (Brain cortex)
Total protein in input 250 µg
Specification Brain cortex
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

提交于 Sep 09 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Gel Running Conditions Reduced Denaturing (10% tris-glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Sep 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain cortex)
Loading amount 10 µg
Specification Brain cortex
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Apr 07 2009

Thank you for your enquiry. Yes, this antibody has been tested for cross-reactivity with both mouse and human. On the online datasheet for ab4150 there are two Western blot images, the first one using Jurkat lysate and the second one showing HeLa whole...

Read More

I'm sorry to hear you are having a problem with our PARK7 (DJ1) antibody ab4150. I would like to suggest the following modifications to your protocol: - The protein subcellular localisation is described as both nuclear and cytoplasmic, I would th...

Read More

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"