Anti-Parathyroid Hormone Receptor 1抗体(ab75150)

概述

  • 产品名称
    Anti-Parathyroid Hormone Receptor 1抗体
    参阅全部 Parathyroid Hormone Receptor 1 一抗
  • 描述
    兔多克隆抗体to Parathyroid Hormone Receptor 1
  • 经测试应用
    适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human, Lizard
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from internal sequence of human Parathyroid Hormone Receptor 1.

  • 阳性对照
    • Human brain tissue. Extracts from Jurkat cells. MCF7 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 66 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能
    Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
  • 组织特异性
    Expressed in most tissues. Most abundant in kidney, bone and liver.
  • 疾病相关
    Jansen metaphyseal chondrodysplasia
    Chondrodysplasia Blomstrand type
    Enchondromatosis multiple
    Eiken skeletal dysplasia
    Primary failure of tooth eruption
  • 序列相似性
    Belongs to the G-protein coupled receptor 2 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC138426 antibody
    • MGC138452 antibody
    • Parathyroid hormone 1 receptor antibody
    • Parathyroid hormone/parathyroid hormone related peptide receptor antibody
    • Parathyroid hormone/parathyroid hormone related protein receptor antibody
    • Parathyroid hormone/parathyroid hormone-related peptide receptor antibody
    • PTH receptor antibody
    • PTH/PTHr receptor antibody
    • PTH/PTHrP receptor antibody
    • PTH/PTHrP type I receptor antibody
    • PTH1 receptor antibody
    • PTH1R antibody
    • PTH1R_HUMAN antibody
    • PTHR 1 antibody
    • PTHR antibody
    • PTHR1 antibody
    see all

图片

  • ab75150 staining Parathyroid Hormone Receptor 1 in Lizard regenerated tail (Anolis sagrei) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 45 minutes at 22°C; antigen retrieval was by heat mediation in sodium citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/200 in 1% horse serum) for 16 hours at 4°C. A Biotin-conjugated horse anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.

    Image is of a regenerated lizard tail cartilage tube, at a proximal region that has begun to ossify. Positive staining was observed in structures resembling embryonic bone collar and early hypertrophic chondrocytes.

    See Abreview

  • Immunohistochemistry analysis of Parathyroid Hormone Receptor 1 in paraffin-embedded human brain tissue using ab75150, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • All lanes : Anti-Parathyroid Hormone Receptor 1 antibody (ab75150) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 66 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 117 kDa,82 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of Parathyroid Hormone Receptor 1 in MCF7 cells using ab75150, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

文献

This product has been referenced in:
  • Hu J  et al. Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling. PLoS One 9:e89352 (2014). WB, ICC/IF ; Mouse . Read more (PubMed: 24551245) »
  • Horie M  et al. Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that enhances expression of type II collagen. Osteoarthritis Cartilage 20:1197-207 (2012). Read more (PubMed: 22750747) »

See all 2 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Kidney, Liver)
Permeabilization
No
Specification
Kidney, Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Daniel Tausan

Verified customer

提交于 Mar 31 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate buffer, pH 6.0
Sample
Lizard Tissue sections (Regenerated lizard tail (Anolis sagrei))
Specification
Regenerated lizard tail (Anolis sagrei)
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Thomas Lozito

Verified customer

提交于 May 06 2014

Thank you for your phone call today and for your questions about using ab75150 in IHC.

Please keep me updated about the results using this antibody after a heat-mediated antigen retrieval with pH 6 citrate buffer. If you need any further assi...

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DISCOUNT CODE: ********Expiration date: 20April 2012 I am very pleased to hear you would like to accept our offer and test ab54186 in cow tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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