Anti-Parathyroid Hormone抗体[D1.1] (ab14498)

Abreviews (1)Q&A (5)

概述

  • 产品名称
    Anti-Parathyroid Hormone抗体[D1.1]
    参阅全部 Parathyroid Hormone 一抗
  • 描述
    小鼠单克隆抗体[D1.1] to Parathyroid Hormone
  • 宿主
    Mouse
  • 经测试应用
    适用于: IHC-P, IHC-Fr, ELISAmore details
  • 种属反应性
    与反应: Dog, Human
    预测可用于: Cow, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    KKEDNVLVESHEKSLGEADKADVNVLTKAKSQ

    , corresponding to amino acids 53-84 of Human Parathyroid Hormone.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    pH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    D1.1
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab14498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ILMA: Use at a concentration of 500 ng/ml.
ELISA Use at an assay dependent concentration. Use at a concentration of 500 ng/ml.

靶标

  • 功能
    PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • 疾病相关
    Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
  • 序列相似性
    Belongs to the parathyroid hormone family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
    • 别名
      • hPTH antibody
      • Parathormone antibody
      • Parathyrin antibody
      • Parathyroid hormone 1 antibody
      • Parathyroid hormone antibody
      • Prepro PTH antibody
      • Preproparathyroid hormone antibody
      • PTH antibody
      • PTH1 antibody
      • PTH1 receptor antibody
      • PTH1R antibody
      • PTHR antibody
      • PTHR1 antibody
      • PTHY_HUMAN antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [D1.1] (ab14498)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [D1.1] (ab14498)Image courtesy of an anonymous Abreview.
      ab14498 staining Parathyroid Hormone in canine parathyroid tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using the microwave method. Samples were then blocked with 10% serum for 20 minutes at room temperature followed by incubation with the primary antibody at a 1/100 dilution for 50 minutes. An undiluted biotin-conjugated goat anti-mouse polyclonal was used as secondary antibody.

    文献

    ab14498 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab14498？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    6 reviews or Q&A

    10
    Votes: Highest First

    As we discussed, please see the attached Nanodrop data regarding the 3 replacement vials you just sent.

    Please let me know if you have any questions.

    Thanks!


    Best Regards,

    Thank you very much for your call today and for forwarding this data.

    I'll keep you updated of the situation and let you know if I find out any more information. Please see below for information about your credit note and refund.

    Yo...

    Read More

    Thanks for following up, please see my end user’s response to your QC process.

    The Bradford assay is a total protein assay, a spectrophotometer to measure protein A280/260 is the proper way to determine the IgG content. Clearly their a...

    Read More

    Thank you very much for forwarding the comments on to me.

    I'm not sure how the IgG concentration can be specifically measured with a spectrophotometer if there were other proteins in the vial. All proteins (containing aromatic residues) will ...

    Read More

    Two vials only contained 20 ug each (should have been 100 ug). How is the mass per vial QC'ed for each new lot?

    Thank you again for all of your patience regarding this issue.

    I've heard back from the lab, and each new lot of antibody is tested after production (i.e. before lyophilization) using a Bradford assay and then is tested again after lyophiliza...

    Read More

    Two vials only contained 20 ug each (should have been 100 ug). How is the mass per vial QC'ed for each new lot?

    Thanks again for all of your patience regarding this issue. I've set up the replacement order, and these vials will arrive on the order *** sometime next week. I will let you know as soon as I have more information from the lab about the QC process for...

    Read More

    Each vial only contained 0.2 mg/mL after reconsitution with 100 uL as instructed. Should have been 1 mg/mL. Concentration measured by calibrated Nanodrop.

    Thank you for your call today and for letting us know about the trouble with ab14498.

    I apologize that I wasn't clear about when I would get back to you. I'll be working with the lab to determine the cause of this problem and fix it, but I'm ...

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Parathyroid Hormone antibody [D1.1]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (parathyroid)
    Specification
    parathyroid
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution (DAKO) pH 6.0, Microwave 2 x 10 min/700 watt
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Apr 23 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册