Anti-Parathyroid Hormone抗体(ab53040)

概述

  • 产品名称Anti-Parathyroid Hormone抗体
    参阅全部 Parathyroid Hormone 一抗
  • 描述
    兔多克隆抗体to Parathyroid Hormone
  • 特异性This antibody detects endogenous levels of total parathyroid hormone
  • 经测试应用适用于: Sandwich ELISA, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from human Parathyroid Hormone corresponding to amino acid 71 to 100.

  • 阳性对照
    • A549 cell extract

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab53040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [A1/70] to Parathyroid Hormone (ab14493). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab14493 as Capture.
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 13 kDa).
ELISA 1/10000.

靶标

  • 功能PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • 疾病相关Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
  • 序列相似性Belongs to the parathyroid hormone family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hPTH antibody
    • Parathormone antibody
    • Parathyrin antibody
    • Parathyroid hormone 1 antibody
    • Parathyroid hormone antibody
    • PTH antibody
    • PTH1 antibody
    • PTH1 receptor antibody
    • PTH1R antibody
    • PTHR antibody
    • PTHR1 antibody
    • PTHY_HUMAN antibody
    see all

Anti-Parathyroid Hormone antibody 图像

实验方案

Anti-Parathyroid Hormone antibody (ab53040)参考文献

ab53040 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

The amino acid range of the immunogen for ab53040 is aa50-100 of human parathyroid hormone with accession number P01270 (http://www.uniprot.org/uniprot/P01270).

If you could provide the exact protein sequen...

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Thank you for your email.

We generally add the location of immunogen e.g. N-terminal, C-terminal or internal on datasheet however I do agree this wasn't the case for this ab53040. This antibody would pick up the whole PTH but not 1-34 aa pepti...

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Thank you for your email.

The immunogen of antibody ab53040 is a sequence from amino acid 71 to aa 100 which might be the reason this antibody is not detecting band of interest.

ab40630 is indeed tested for 100% reactivity with 1-34 p...

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Thank you very much for your reply and for the information.
I have added a vial of ab40630 to a shipment and forwarded the details of this replacement to your contact.
I will be back in touch regarding ab53040, and if there is anything else th...

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Thank you very much for your reply and for sending the information about the PTHrP expression in A549 cells.
I will follow-up with the lab about ab53040, and I am happy to send a vial of ab40630. I will need to send this vial first to Zotal and th...

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Thank you for your reply.
I can send one of the rabbit polyclonal antibodies ab40630 or ab53040, but their immunogens have lower homology to the rat protein so they may show a weaker cross-reactivity. I certainly understand that a rabbit polyclona...

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Thank you for your reply.

Would you please provide the end user information so that we can confirm the Abreview submission? Thank you in advance.

I am very pleased to hear you would like to accept our offer and test ab111097 in WB...

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Thank you for contacting Abcam regarding ab53040.

I have aligned the immunogen sequence with the mouse sequence and unfortunately, there is only 60% homology between the two. We would not predict that this antibody will cross react with the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"