Anti-Parathyroid Hormone抗体(ab111097)

概述

  • 产品名称
    Anti-Parathyroid Hormone抗体
    参阅全部 Parathyroid Hormone 一抗
  • 描述
    兔多克隆抗体to Parathyroid Hormone
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide, derived from an internal sequence of Human Parathyroid Hormone (UniProt ID: P01270).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Constituent: Whole serum
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/500.
WB 1/200 - 1/2000. Predicted molecular weight: 13 kDa.

靶标

  • 功能
    PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • 疾病相关
    Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
  • 序列相似性
    Belongs to the parathyroid hormone family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hPTH antibody
    • Parathormone antibody
    • Parathyrin antibody
    • Parathyroid hormone 1 antibody
    • Parathyroid hormone antibody
    • Prepro PTH antibody
    • Preproparathyroid hormone antibody
    • PTH antibody
    • PTH1 antibody
    • PTH1 receptor antibody
    • PTH1R antibody
    • PTHR antibody
    • PTHR1 antibody
    • PTHY_HUMAN antibody
    see all

Anti-Parathyroid Hormone antibody (ab111097)参考文献

ab111097 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your reply and for the information.
I have added a vial of ab40630 to a shipment and forwarded the details of this replacement to your contact.
I will be back in touch regarding ab53040, and if there is anything else th...

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Thank you very much for your reply and for sending the information about the PTHrP expression in A549 cells.
I will follow-up with the lab about ab53040, and I am happy to send a vial of ab40630. I will need to send this vial first to Zotal and th...

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Thank you for your reply.
I can send one of the rabbit polyclonal antibodies ab40630 or ab53040, but their immunogens have lower homology to the rat protein so they may show a weaker cross-reactivity. I certainly understand that a rabbit polyclona...

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Thank you for sending the images and the extra information.
After seeing the images, I agree that the antibody is not binding to the PTH protein as it should be. I would be happy to send a replacement antibody if you would like. We have a few othe...

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Application
Western blot
Sample
Human Cell lysate - whole cell (transfected HEK293 cells)
Loading amount
250000 cells
Specification
transfected HEK293 cells
Gel Running Conditions
Reduced Denaturing (12-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Jun 06 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (parathyroid glands)
Loading amount
10 µg
Specification
parathyroid glands
Gel Running Conditions
Reduced Denaturing (12-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Jun 06 2012

Thank you for taking the time to submit this further information.

I appreciate the time the customer has spent in the laboratory and understand their concerns. It is regrettable the results have not been successful. I would like to make some...

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Thank you for contacting us.

I am sorry to hear your customer has been experiencing problems with one of our Parathyroid Hormone antibodies. The quality of our products is important to us and I would like to reassure you that we investigate ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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