Parainfluenza Virus type 1, all structural antigens. Recognises Sendai virus by ELISA. Does not cross-react with Para types 2 and 3, Influenza A, Influenza B, Respiratory Syncytial Virus, HSV1, HSV2, Adenovirus, CMV, Measles, Mumps and Rubella by indirect IFA. Does not react with HEP2 cells or monkey kidney cells by indirect IFA.
Purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed. Estimated HRP:IgG substitution is 2-3.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/200 - 1/1000.
Human parainfluenza viruses (HPIV) were first discovered in the late 1950s. HPIV is genetically and antigenically divided into types 1 to 4. HPIV 1 to HPIV 3 are major causes of lower respiratory infections in infants, young children, the immunocompromised, the chronically ill, and the elderly. Each subtype can cause somewhat unique clinical diseases in different hosts. HPIV are enveloped and of medium size (150 to 250 nm), and their RNA genome is in the negative sense. These viruses belong to the Paramyxoviridae family, one of the largest and most rapidly growing groups of viruses causing significant human and veterinary disease. HPIV are closely related to recently discovered megamyxoviruses (Hendra and Nipah viruses) and metapneumovirus.