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Our Abpromise guarantee covers the use of ab5787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).Can be blocked with PAR4 peptide (ab5853).|
|IP||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. PubMed: 19801652|
|ICC/IF||Use a concentration of 5 µg/ml.|
ICC/IF image of ab5787 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab5787 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Shows a Western blot of PAR4 on rat spleen extract using ab5787.
Immunohistochemical analysis of paraffin-embedded mouse embryonic corneal tissue, staining PAR4 with ab5787.
Embryos were fixed with 4% paraformaldehyde and treated for antigen retrieval by boiling for 10 min in the citrate buffer (pH 6.0). Sections were incubated with primary antibody and a biotinylated anti-rabbit secondary antibody.