概述

  • 产品名称Anti-PAQR5抗体
  • 描述
    兔多克隆抗体to PAQR5
  • 经测试应用适用于: ICC, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Sheep, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from the C terminal of Human PAQR5

  • 阳性对照
    • Extracts from HUVEC cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ELISA 1/5000.

靶标

  • 相关性PAQR5 (Progestin and adipoQ receptor family member V) is a steroid membrane receptor which binds progesterone. It may be involved in oocyte maturation. It is an integral membrane protein and is expressed in kidney, colon, adrenal gland and lung. It belongs to the ADIPOR family.
  • 细胞定位Membrane; Multi-pass membrane protein.
  • 数据库链接
  • 别名
    • FLJ20190 antibody
    • Membrane progestin receptor gamma antibody
    • mPR gamma antibody
    • MPRG antibody
    • PAQR 5 antibody
    • Progestin and adipoQ receptor family member V antibody
    see all

Anti-PAQR5 antibody 图像

  • All lanes : Anti-PAQR5 antibody (ab79517) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HUVEC cells with mmunizing peptide at 10 µg

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 38 kDa
    Observed band size : 38 kDa
  • All lanes : Anti-PAQR5 antibody (ab79517) at 500 µg

    Lane 1 : HUVEC cell lysate (positive control)
    Lane 2 : Non-pregnant ovine caruncle tissue lysate
    Lane 3 : Non-pregnant ovine caruncle tissue lysate
    Lane 4 : Non-pregnant ovine caruncle tissue lysate
    Lane 5 : Day 30 of gestation ovine caruncle tissue lysate
    Lane 6 : Day 30 of gestation ovine caruncle tissue lysate
    Lane 7 : Day 30 of gestation ovine caruncle tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa (possible non-specific binding).

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab79517 staining PAQR5 in murine ovary tissue by Immunohistochemistry (PFA-fixed).
    Tissue was fixed in paraformaldehyde and blocked using 10% normal goat serum for 1 hour at 25°C, then incubated with ab79517 at a 1/500 dilution for 12 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.
    ab79517 staining PAQR5 in murine ovary tissue by Immunohistochemistry (PFA-fixed).
    Tissue was fixed in paraformaldehyde and blocked using 10% normal goat serum for 1 hour at 25°C, then incubated with ab79517 at a 1/500 dilution for 12 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

Anti-PAQR5 antibody (ab79517)参考文献

This product has been referenced in:
  • Bashour NM & Wray S Progesterone directly and rapidly inhibits GnRH neuronal activity via progesterone receptor membrane component 1. Endocrinology 153:4457-69 (2012). Read more (PubMed: 22822163) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10 %)
Sample Sheep Purified protein (caruncle tissue)
Specification caruncle tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Oct 09 2014

Thank you for contacting Abcam. The immunogen peptide for ab75508 is is from *******and for ab79517, the immnuogen peptide is from ******, both peptides are from the human sequence. If there is anything else I can help you with, please let me know.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cell (Mouse ovary sections)
Specification Mouse ovary sections
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mr. Nicholas Bashour

Verified customer

提交于 Dec 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"