• 产品名称Anti-PAPSS1抗体
    参阅全部 PAPSS1 一抗
  • 描述
    小鼠单克隆抗体to PAPSS1
  • 经测试应用适用于: WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 144-253 of Human PAPSS1


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab56398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

ICC/IF 1/250.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • 功能Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.
  • 组织特异性Expressed in testis, pancreas, kidney, thymus, prostate, ovary, small intestine, colon, leukocytes and liver. Also expressed in high endothelial venules (HEV) cells and in cartilage.
  • 通路Sulfur metabolism; sulfate assimilation.
  • 序列相似性In the N-terminal section; belongs to the APS kinase family.
    In the C-terminal section; belongs to the sulfate adenylyltransferase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3 prime phosphoadenosine 5 prime phosphosulfate synthase 1 antibody
    • 3' phosphoadenosine 5' phosphosulfate synthase 1 antibody
    • 3''-phosphoadenosine-5''-phosphosulfate synthase antibody
    • Adenosine-5''-phosphosulfate 3''-phosphotransferase antibody
    • Adenylyl-sulfate kinase antibody
    • Adenylylsulfate 3''-phosphotransferase antibody
    • APS kinase antibody
    • ATP-sulfurylase antibody
    • ATPSK 1 antibody
    • ATPSK1 antibody
    • ATPSK2 antibody
    • Bifunctional 3' phosphoadenosine 5' phosphosulfate synthetase 1 antibody
    • PAPS synthase 1 antibody
    • PAPS synthetase 1 antibody
    • PAPS1_HUMAN antibody
    • PAPSS 1 antibody
    • PAPSS antibody
    • PAPSS1 antibody
    • SAT antibody
    • SK 1 antibody
    • SK1 antibody
    • SK2 antibody
    • Sulfate adenylate transferase antibody
    • Sulfurylase kinase 1 antibody
    see all

Anti-PAPSS1 antibody 图像

  • Overlay histogram showing HeLa cells stained with ab56398 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56398, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot against tagged recombinant protein immunogen using ab56398 PAPSS1 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa

Anti-PAPSS1 antibody (ab56398)参考文献

ab56398 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Adreanl Carcinoma)
Specification Adreanl Carcinoma
Fixative Methanol
Permeabilization Yes - 1% TX100

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提交于 Oct 06 2009