概述

  • 产品名称Anti-PAPP A抗体[18A10]
    参阅全部 PAPP A 一抗
  • 描述
    小鼠单克隆抗体[18A10] to PAPP A
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified human PAPPA antigen

  • 常规说明Concentration varies from lot to lot and can be provided on request.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Purified by chromatography on protein A. Purity tested by electrophoresis
  • 克隆单克隆
  • 克隆编号18A10
  • 骨髓瘤Sp2/0
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 200 kDa under reducing conditions. (predicted molecular weight: 195 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
    • 组织特异性High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.
    • 序列相似性Belongs to the peptidase M43B family.
      Contains 5 Sushi (CCP/SCR) domains.
    • 发展阶段Present in serum and placenta during pregnancy; levels increase throughout pregnancy.
    • 翻译后修饰There appear to be no free sulfhydryl groups.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ASBABP2 antibody
      • Aspecific BCL2 ARE binding protein 2 antibody
      • Differentially placenta 1 expressed protein antibody
      • DIPLA1 antibody
      • EC 3.4.24.79 antibody
      • IGF dependent IGFBP 4 protease antibody
      • IGF-dependent IGFBP-4 protease antibody
      • IGFBP 4ase antibody
      • IGFBP-4ase antibody
      • IGFBP4ase antibody
      • Insulin like growth factor dependent IGF binding protein 4 protease antibody
      • Insulin-like growth factor-dependent IGF-binding protein 4 protease antibody
      • PAPA antibody
      • PAPP A antibody
      • PAPP-A antibody
      • PAPP1_HUMAN antibody
      • PAPPA antibody
      • PAPPA1 antibody
      • Pappalysin 1 precursor antibody
      • Pappalysin-1 antibody
      • Pregnancy Associated Plasma Protein A antibody
      • Pregnancy associated plasma protein A pappalysin 1 antibody
      • Pregnancy-associated plasma protein A antibody
      see all

    Anti-PAPP A antibody [18A10] 图像



    • Predicted band size : 195 kDa


      Detection of human PAPP-A by ab10196 in Western Blotting. A. ab10196 recognizes PAPP-A subunit after SDS-gel electrophoresis in reducing conditions (gradient gel 5 % – 15 %) B. ab10196 recognizes heterotetrameric complex (gradient gel 2.5 % – 10 %)

    Anti-PAPP A antibody [18A10] (ab10196)参考文献

    This product has been referenced in:
    • Escamilla-Gómez V  et al. Simultaneous detection of free and total prostate specific antigen on a screen-printed electrochemical dual sensor. Biosens Bioelectron 24:2678-83 (2009). Human . Read more (PubMed: 19261459) »

    See 1 Publication for this product

    Product Wall

    The concentration of the lot 74368 is 3.4 mg/ml. You can use the antibody with a final concentration of 10-20 ug/ml in WB. The optimal concentration needs to be determined separately for every application.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"