概述

  • 产品名称
    Anti-PAPP A抗体[004-09]
    参阅全部 PAPP A 一抗
  • 描述
    小鼠单克隆抗体[004-09] to PAPP A
  • 特异性
    This antibody is specific for native human PAPP A. Heat and SDS denatured PAPP A is also recognized, but not acid treated PAPP A.
  • 经测试应用
    适用于: WB, ELISA, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) (Human)

  • 表位
    The epitope is located on the PAPP A subunit of the heterotetramer.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯化说明
    Protein A/G purified
  • 克隆
    单克隆
  • 克隆编号
    004-09
  • 同种型
    IgG2b
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 181 kDa.
ELISA 1/8000. This antibody can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP A in coat.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
  • 组织特异性
    High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.
  • 序列相似性
    Belongs to the peptidase M43B family.
    Contains 5 Sushi (CCP/SCR) domains.
  • 发展阶段
    Present in serum and placenta during pregnancy; levels increase throughout pregnancy.
  • 翻译后修饰
    There appear to be no free sulfhydryl groups.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ASBABP2 antibody
    • Aspecific BCL2 ARE binding protein 2 antibody
    • Differentially placenta 1 expressed protein antibody
    • DIPLA1 antibody
    • EC 3.4.24.79 antibody
    • IGF dependent IGFBP 4 protease antibody
    • IGF-dependent IGFBP-4 protease antibody
    • IGFBP 4ase antibody
    • IGFBP-4ase antibody
    • IGFBP4ase antibody
    • Insulin like growth factor dependent IGF binding protein 4 protease antibody
    • Insulin-like growth factor-dependent IGF-binding protein 4 protease antibody
    • PAPA antibody
    • PAPP A antibody
    • PAPP-A antibody
    • PAPP1_HUMAN antibody
    • PAPPA antibody
    • PAPPA1 antibody
    • Pappalysin 1 precursor antibody
    • Pappalysin-1 antibody
    • Pregnancy Associated Plasma Protein A antibody
    • Pregnancy associated plasma protein A pappalysin 1 antibody
    • Pregnancy-associated plasma protein A antibody
    see all

图片

  • Ab52030 staining human PAPP A in human placenta tissue by immunohistochemistry using frozen tissue. Staining (red-brown) is restricted to the syncytiotrophoblast of the chorionic villi.
  • Ab52030 staining human PAPP A in human placenta tissue by immunohistochemistry using parrafin embedded tissue. Staining (red-brown) is restricted to the syncytiotrophoblast of the chorionic villi.

文献

This product has been referenced in:
  • Sun Y  et al. MiR-490-3p modulates the proliferation of vascular smooth muscle cells induced by ox-LDL through targeting PAPP-A. Cardiovasc Res 100:272-9 (2013). Read more (PubMed: 23821525) »

See 1 Publication for this product

客户评价及客户问答

Thank you very much for your call today, and I am very sorry for the delay in getting back to you.

I'm sending a free of charge vial of ab52030 on the order ***, which should arrive tomorrow.

Please keep us updated about the results...

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Thank you for your inquiry.

ab59086: Five different pappalysin-1 sequences are published thus far; 1628, 1627, 1420, 1232 and 704 amino acids in length. The shorter forms start with the second methionine, 209 residues downstream from the long...

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Thank you for your reply.

Iam sorry to confirm thatwe do not have the requested information.

The immunogen used to raise ab52030 was PAPP-A purified from fluid containing maternal serum aspirated during cesarean section (“cesa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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