• 产品名称
  • 描述
    兔多克隆抗体to PAPOLA
  • 经测试应用
    适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between residue 694 and the C-terminus (residue 744) of human PAPOLA (NP_116021.2)

  • 阳性对照
    • HeLa whole cell lysate.


  • 形式
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab72492 was affinity purified using an epitope specific to PAPOLA immobilized on solid support.
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab72492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
    • 序列相似性
      Belongs to the poly(A) polymerase family.
    • 翻译后修饰
      Polysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity.
      Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF.
      Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex.
    • 细胞定位
      Cytoplasm. Nucleus. The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic.
    • Information by UniProt
    • 数据库链接
    • 别名
      • MGC5378 antibody
      • PAP alpha antibody
      • PAP antibody
      • PAP-alpha antibody
      • PapIII antibody
      • PAPOA_HUMAN antibody
      • PAPOLA antibody
      • Plap antibody
      • Poly(A) polymerase alpha antibody
      • Polynucleotide adenylyltransferase alpha antibody
      see all

    Anti-PAPOLA antibody 图像

    • All lanes : Anti-PAPOLA antibody (ab72492) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Predicted band size : 83 kDa
      Observed band size : 83 kDa

      Exposure time : 3 minutes


    Anti-PAPOLA antibody (ab72492)参考文献

    ab72492 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for the protocol and result details. Regarding a replacement, there have not been other complaints about the antibody but not many people have purchased it. We have only the lot that you received, and I am concerned that another vial will sim...

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    Thank you for your reply. I have found your order in our system.

    Could you please let me know also the following details:

    1) What antibody dilution and incubation time was used?
    2) What was used for blocking?
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