概述

  • 产品名称Anti-PAPOLA抗体
    参阅全部 PAPOLA 一抗
  • 描述
    兔多克隆抗体to PAPOLA
  • 经测试应用适用于: IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Reptiles, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residues 525 and 575 of human PAPOLA (NP_116021.2)

  • 阳性对照
    • HeLa whole cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab72426 was affinity purified using an epitope specific to PAPOLA immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab72426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
    • 序列相似性Belongs to the poly(A) polymerase family.
    • 翻译后修饰Polysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity.
      Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF.
      Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex.
    • 细胞定位Cytoplasm. Nucleus. The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic.
    • Information by UniProt
    • 数据库链接
    • 别名
      • MGC5378 antibody
      • PAP alpha antibody
      • PAP antibody
      • PAP-alpha antibody
      • PapIII antibody
      • PAPOA_HUMAN antibody
      • PAPOLA antibody
      • Plap antibody
      • Poly(A) polymerase alpha antibody
      • Polynucleotide adenylyltransferase alpha antibody
      see all

    Anti-PAPOLA antibody (ab72426)参考文献

    ab72426 has not yet been referenced specifically in any publications.

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