Anti-Papillomavirus Regulatory Factor 1 / HDBP2抗体(ab75727)

概述

  • 产品名称Anti-Papillomavirus Regulatory Factor 1 / HDBP2抗体
    参阅全部 Papillomavirus Regulatory Factor 1 / HDBP2 一抗
  • 描述
    兔多克隆抗体to Papillomavirus Regulatory Factor 1 / HDBP2
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human) from the center region of Papillomavirus Regulatory Factor 1/ HDBP2, conjugated to KLH.

  • 阳性对照
    • Y79 cell lysate Human hepatocellular carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab75727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Predicted molecular weight: 55 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Plays a role in papillomavirus genes transcription.
  • 组织特异性Widely expressed.
  • 序列相似性Contains 1 C2H2-type zinc finger.
  • 细胞定位Cytoplasm. Nucleus. May shuttle between nucleus and cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp434K1210 antibody
    • HD gene regulatory region binding protein 2 antibody
    • HD gene regulatory region-binding protein 2 antibody
    • HD-regulating factor 2 antibody
    • HDBP-2 antibody
    • HDBP2 antibody
    • HDRF-2 antibody
    • Huntington disease gene regulatory region-binding protein 2 antibody
    • Huntington's disease gene regulatory region binding protein 2 antibody
    • Papillomavirus regulatory factor 1 antibody
    • papillomavirus regulatory factor PRF 1 antibody
    • Papillomavirus-binding factor antibody
    • PBF antibody
    • PRF-1 antibody
    • PRF1 antibody
    • PTTG1IP antibody
    • Si-1-8-14 antibody
    • Zinc finger protein 395 antibody
    • ZN395_HUMAN antibody
    • znf395 antibody
    see all

Anti-Papillomavirus Regulatory Factor 1 / HDBP2 antibody 图像

  • All lanes : Anti-Papillomavirus Regulatory Factor 1 / HDBP2 antibody (ab75727) at 1/100 dilution

    Lane 1 : Human renal cancer whole cell lysate
    Lane 2 : Human renal cancer whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (ab186692)

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55-60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa (possible non-specific binding),37 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-Papillomavirus Regulatory Factor 1 / HDBP2 antibody (ab75727) at 1/60 dilution + Y79 cell lysate at 35 µg

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 37 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue labelled with ab75727 at 1/50 dilution, followed by a peroxidase conjugated secondary antibody and DAB staining.

Anti-Papillomavirus Regulatory Factor 1 / HDBP2 antibody (ab75727)参考文献

ab75727 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12% acrylamide)
Sample Human Cell lysate - whole cell (Renal cancer cell line)
Specification Renal cancer cell line
Blocking step Li-cor Blocking Buffer PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"