Anti-Papillomavirus Regulatory Factor 1 / HDBP2抗体(ab75727)

概述

  • 产品名称
    Anti-Papillomavirus Regulatory Factor 1 / HDBP2抗体
    参阅全部 Papillomavirus Regulatory Factor 1 / HDBP2 一抗
  • 描述
    兔多克隆抗体to Papillomavirus Regulatory Factor 1 / HDBP2
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human) from the center region of Papillomavirus Regulatory Factor 1/ HDBP2, conjugated to KLH.

  • 阳性对照
    • Y79 cell lysate Human hepatocellular carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab75727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Predicted molecular weight: 55 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

靶标

  • 功能
    Plays a role in papillomavirus genes transcription.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Contains 1 C2H2-type zinc finger.
  • 细胞定位
    Cytoplasm. Nucleus. May shuttle between nucleus and cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp434K1210 antibody
    • HD gene regulatory region binding protein 2 antibody
    • HD gene regulatory region-binding protein 2 antibody
    • HD-regulating factor 2 antibody
    • HDBP-2 antibody
    • HDBP2 antibody
    • HDRF-2 antibody
    • Huntington disease gene regulatory region-binding protein 2 antibody
    • Huntington's disease gene regulatory region binding protein 2 antibody
    • Papillomavirus regulatory factor 1 antibody
    • papillomavirus regulatory factor PRF 1 antibody
    • Papillomavirus-binding factor antibody
    • PBF antibody
    • PRF-1 antibody
    • PRF1 antibody
    • PTTG1IP antibody
    • Si-1-8-14 antibody
    • Zinc finger protein 395 antibody
    • ZN395_HUMAN antibody
    • znf395 antibody
    see all

图片

  • All lanes : Anti-Papillomavirus Regulatory Factor 1 / HDBP2 antibody (ab75727) at 1/100 dilution

    Lane 1 : Human renal cancer whole cell lysate
    Lane 2 : Human renal cancer whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (ab186692)

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55-60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa (possible non-specific binding),37 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-Papillomavirus Regulatory Factor 1 / HDBP2 antibody (ab75727) at 1/60 dilution + Y79 cell lysate at 35 µg

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 37 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue labelled with ab75727 at 1/50 dilution, followed by a peroxidase conjugated secondary antibody and DAB staining.

文献

ab75727 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12% acrylamide)
Sample
Human Cell lysate - whole cell (Renal cancer cell line)
Specification
Renal cancer cell line
Blocking step
Li-cor Blocking Buffer PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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提交于 Jul 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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