概述

  • 产品名称Anti-PAPD4抗体
  • 描述
    兔多克隆抗体to PAPD4
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Hamster, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 65-95 of Human PAPD4.

  • 阳性对照
    • MCF-7 and CHO cell lysates; Mouse heart tissue lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab103884 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab103884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/1000. Predicted molecular weight: 56 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 23700402

靶标

  • 功能Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation.
  • 组织特异性Expressed in brain. Within brain, it is expressed in cerebellum, hippocampus and medulla.
  • 序列相似性Belongs to the DNA polymerase type-B-like family. GLD2 subfamily.
    Contains 1 PAP-associated domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 8030446C20Rik antibody
    • AI649009 antibody
    • FLJ38499 antibody
    • GLD 2 antibody
    • Gld2 antibody
    • GLD2_HUMAN antibody
    • hGLD 2 antibody
    • hGLD-2 antibody
    • MGC93684 antibody
    • PAD 4 antibody
    • PAP associated domain containing 4 antibody
    • PAP-associated domain-containing protein 4 antibody
    • papd4 antibody
    • Poly(A) RNA polymerase GLD2 antibody
    • Terminal uridylyltransferase 2 antibody
    • TUTase 2 antibody
    see all

Anti-PAPD4 antibody 图像

  • All lanes : Anti-PAPD4 antibody (ab103884) at 1/1000 dilution

    Lane 1 : MCF-7 whole cell lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : CHO whole cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 56 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-PAPD4 antibody (ab103884)参考文献

This product has been referenced in:
  • de Sousa É  et al. Developmental and functional expression of miRNA-stability related genes in the nervous system. PLoS One 8:e56908 (2013). WB, IHC-P ; Rat . Read more (PubMed: 23700402) »
  • Plé H  et al. The repertoire and features of human platelet microRNAs. PLoS One 7:e50746 (2012). WB ; Human . Read more (PubMed: 23226537) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (liver carcinoma cell line)
Specification liver carcinoma cell line
Treatment siRNA(PAPD4) for 48 h
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Nov 05 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"