概述

  • 产品名称Anti-Pannexin 1抗体[EPR5556]
    参阅全部 Pannexin 1 一抗
  • 描述
    兔单克隆抗体[EPR5556] to Pannexin 1
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Pannexin 1 aa 350 to the C-terminus. The exact sequence is proprietary.

  • 阳性对照
    • WB: Human fetal brain tissue lysate and SH-SY5Y, Caco2, and NIH OVCAR-3 cell lysates. IP: SH-SY5Y cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 解离常数(KD KD = 1.52 x 10 -10 M Learn more about KD
  • 存储溶液pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号EPR5556
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/10 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    • 功能Structural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis.
    • 序列相似性Belongs to the pannexin family.
    • 细胞定位Cell membrane. Cell junction > gap junction. Endoplasmic reticulum membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • innexin antibody
      • MRS1 antibody
      • Pannexin 1 antibody
      • Pannexin-1 antibody
      • Panx1 antibody
      • PANX1_HUMAN antibody
      • PX1 antibody
      • UNQ2529 antibody
      see all

    Anti-Pannexin 1 antibody [EPR5556] 图像

    • All lanes : Anti-Pannexin 1 antibody [EPR5556] (ab124969) at 1/10000 dilution (purified)

      Lane 1 : Caco-2 whole cell lysate
      Lane 2 : NIH:OVCAR-3 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 48 kDa
      Observed band size : 48 kDa

      Blocking and dilution buffer: 5% NFDM /TBST.

    • ab124969 (purified) at 1/20 immunoprecipitating Pannexin 1 in SH-SY5Y whole cell lysate.

      Lane 1 (input): SH-SY5Y whole cell lysate (10µg)

      Lane 2 (+): ab124969 + SH-SY5Y whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124969 in SH-SY5Y whole cell lysate.

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Pannexin 1 antibody [EPR5556] (ab124969) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal brain cell lysates
      Lane 2 : SH-SY5Y cell lysates
      Lane 3 : Caco2 cell lysates
      Lane 4 : NIH OVCAR-3 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 48 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Pannexin 1 antibody [EPR5556] (ab124969)参考文献

    This product has been referenced in:
    • Skals M  et al. Bacterial RTX toxins allow acute ATP release from human erythrocytes directly through the toxin pore. J Biol Chem 289:19098-109 (2014). WB ; Mouse . Read more (PubMed: 24860098) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"