概述

  • 产品名称Anti-PANK1抗体
    参阅全部 PANK1 一抗
  • 描述
    小鼠多克隆抗体to PANK1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human PANK1 protein (NP_683879.1)

  • 阳性对照
    • PANK1 transfected 293T cell lysate and human liver tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab69354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Detects band of approximately 37 and 50 kDa in two separate blots (predicted molecular weight for isoform 2: 41 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Plays a role in the physiological regulation of the intracellular CoA concentration.
    • 组织特异性Isoform 1 is expressed at high levels in brain, heart, kidney, liver, skeletal muscle and testis. Isoform 2 is detected at much lower levels in kidney, liver, brain and testis and is not detected in heart or skeletal muscle.
    • 通路Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.
    • 序列相似性Belongs to the type II pantothenate kinase family.
    • 结构域The N-terminal extension, present in isoform 1 may be the regulatory domain.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • coaA antibody
      • hPanK antibody
      • hPanK1 antibody
      • PANK antibody
      • PANK1 antibody
      • PANK1_HUMAN antibody
      • PANK1a antibody
      • PANK1b antibody
      • PanK1beta antibody
      • Pantothenate kinase 1 antibody
      • Pantothenate kinase antibody
      • Pantothenic acid kinase 1 antibody
      see all

    Anti-PANK1 antibody 图像

    • Anti-PANK1 antibody (ab69354) at 1/500 dilution + human liver tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 41 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-PANK1 antibody (ab69354) at 1/500 dilution

      Lane 1 : PANK1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 41 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    Anti-PANK1 antibody (ab69354)参考文献

    ab69354 has not yet been referenced specifically in any publications.

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