Anti-Pancreatic Polypeptide抗体(ab77192)
Key features and details
- Goat polyclonal to Pancreatic Polypeptide
- Suitable for: IHC-P, ELISA, ICC/IF, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Pancreatic Polypeptide抗体
参阅全部 Pancreatic Polypeptide 一抗 -
描述
山羊多克隆抗体to Pancreatic Polypeptide -
宿主
Goat -
经测试应用
适用于: IHC-P, ELISA, ICC/IF, WB, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Dog, Chimpanzee, Macaque monkey -
免疫原
Synthetic peptide corresponding to Human Pancreatic Polypeptide aa 61-73 (internal sequence).
Sequence:TRPRYGKRHKEDT
Database link: P01298 -
阳性对照
- IHC-P: Human pancreas tissue. Flow Cyt (intra): U2OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77192于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (2) |
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. |
ELISA |
Use at an assay dependent concentration.
Antibody detection limit dilution 1:32,000 by peptide ELISA. |
|
ICC/IF | (1) |
Use at an assay dependent concentration.
See Abreview. |
WB |
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.
Preliminary experiments gave an approx 35-38 kDa band in human pancreas lysate, which was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5 kDa. |
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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说明 |
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IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. |
ELISA
Use at an assay dependent concentration. Antibody detection limit dilution 1:32,000 by peptide ELISA. |
ICC/IF
Use at an assay dependent concentration. See Abreview. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa. Preliminary experiments gave an approx 35-38 kDa band in human pancreas lysate, which was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5 kDa. |
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
靶标
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相关性
Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. -
细胞定位
Secreted -
数据库链接
- Entrez Gene: 5539 Human
- Entrez Gene: 713988 Macaque monkey
- Entrez Gene: 19064 Mouse
- Entrez Gene: 24677 Rat
- Omim: 167780 Human
- SwissProt: P01298 Human
- SwissProt: P33684 Macaque monkey
- SwissProt: P10601 Mouse
see all -
别名
- Pancreatic polypeptide antibody
- Pancreatic polypeptide Y antibody
- Pancreatic prohormone antibody
see all
图片
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Staining of formalin-fixed, paraffin-embedded human pancreas tissue with 3µg/ml ab77192. Note strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans.
Microwaved antigen retrieval with Tris/EDTA buffer pH6, HRP-staining.
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Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
实验方案
数据表及文件
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Datasheet download
文献 (20)
ab77192 被引用在 20 文献中.
- Yu XX et al. Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans. Cell Res 31:886-903 (2021). PubMed: 33692492
- Zhao H et al. Pre-existing beta cells but not progenitors contribute to new beta cells in the adult pancreas. Nat Metab 3:352-365 (2021). PubMed: 33723463
- Wu M et al. Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry. Cell Rep 37:109919 (2021). PubMed: 34731614
- Brioudes E et al. Syndecan-4 is regulated by IL-1ß in ß-cells and human islets. Mol Cell Endocrinol 510:110815 (2020). PubMed: 32315719
- Yamauchi Y et al. Rb and p53 Execute Distinct Roles in the Development of Pancreatic Neuroendocrine Tumors. Cancer Res 80:3620-3630 (2020). PubMed: 32591410