Anti-Pancreatic Polypeptide抗体(ab77192)


  • 产品名称Anti-Pancreatic Polypeptide抗体
    参阅全部 Pancreatic Polypeptide 一抗
  • 描述
    山羊多克隆抗体to Pancreatic Polypeptide
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Dog, Chimpanzee, Macaque monkey
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 61-73 of Human Pancreatic Polypeptide

  • 阳性对照
    • Human pancreas


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab77192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 10 kDa).

Preliminary experiments gave an approx 35-38 kDa band in human pancreas lysate, which was successfully blocked by incubation with the immunizing peptide.  Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5 kDa.


ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:32,000 by peptide ELISA.

IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans.

ICC/IF 1/150.

See Abreview


  • 相关性Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • Pancreatic polypeptide antibody
    • Pancreatic polypeptide Y antibody
    • Pancreatic prohormone antibody
    • PNP antibody
    • PP antibody
    • PPY antibody
    • prepro-PP (prepropancreatic polypeptide) antibody
    see all

Anti-Pancreatic Polypeptide antibody 图像

  • Staining of formalin-fixed, paraffin-embedded Human pancreas tissue with 3µg/ml ab77192. Note strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans.

Anti-Pancreatic Polypeptide antibody (ab77192)参考文献

This product has been referenced in:
  • Ackermann AM  et al. Integration of ATAC-seq and RNA-seq identifies human alpha cell and beta cell signature genes. Mol Metab 5:233-44 (2016). Human . Read more (PubMed: 26977395) »
  • Wang YJ  et al. Single-Cell Mass Cytometry Analysis of the Human Endocrine Pancreas. Cell Metab 24:616-626 (2016). Alexa Fluor® 568 ; Human . Read more (PubMed: 27732837) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Pancreas)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer pH6.0
Specification Pancreas
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rm°C
Fixative Formaldehyde

Jing Ma

Verified customer

提交于 Mar 28 2016

Thank you for contacting us.

Unfortunately this lot is sold out so we can not offer this - we however have new lot in sock.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Pancreas)
Specification Pancreas
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No

Abcam user community

Verified customer

提交于 Nov 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Pancreas)
Specification Pancreas
Fixative Paraformaldehyde
Permeabilization Yes - PBSx1/ 0.1% TritonX100
Blocking step No blocking step used for 30 minute(s) · Temperature: 25°C

Abcam user community

Verified customer

提交于 Oct 19 2011