Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Pan3.
- This antibody gave a positive signal in the following lysates:
HeLa Whole Cell; HepG2 Whole Cell; MOLT4 Whole Cell; Jurkat Whole Cell; Human Liver Tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 82 kDa).
Functions in cytoplasmic mRNA decay. As part of the Pan nuclease complex, recruits polyadenylate-binding protein which in turn stimulates PAN2 nuclease activity.
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
The protein kinase domain is predicted to be catalytically inactive.
Information by UniProt
- hPan3 antibody
- PAB dependent poly(A) specific ribonuclease subunit 3 antibody
- PAB-dependent poly(A)-specific ribonuclease subunit 3 antibody
Western blot - Pan3 antibody (ab88642)
All lanes :
Anti-Pan3 antibody (ab88642) at 1 µg/mlLane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 :
MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLane 4 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 5 :
Human liver tissue lysate - total protein (ab29889
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
82 kDaObserved band size:
80 kDa (why is the actual band size different from the predicted?
)Additional bands at:
160 kDa, 60 kDa. We are unsure as to the identity of these extra bands.Exposure time:
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"