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cell preparation: Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells).
Our Abpromise guarantee covers the use of ab20740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab20740 at a 1/50 dilution staining human U2 OS cells by Immunocytochemistry/Immunofluorescence. The cells were Paraformaldehyde fixed and incubated with the antibody for 16 hours. Bound antibody was detected using an Alexa- Fluor ® 488 conjugated goat anti-mouse IgG.
The antibody was found to stain equally well when used at a dilution of 1/250.
This image is courtesy of an Abreview submitted by Chi Tang on 6 April 2006.