Anti-pan Keratin抗体[80] (ab8068)

概述

  • 产品名称Anti-pan Keratin抗体[80]
    参阅全部 pan Keratin 一抗
  • 描述
    小鼠单克隆抗体[80] to pan Keratin
  • 特异性In immunoblots most human keratins are recognized. No reactivity with neurofilament, vimentin GFAP or Desmin
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Callus cytokeratins isolated from fresh human skin tissue

  • 阳性对照
    • Human callus or keratinocytes

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.07% Sodium azide
    Constituent: 1% Fetal calf serum
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号80
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/20. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration. Stains all types of keratin containing cells (epithelia) in frozen sections of various tissues, with the exception of myoepithelial cells.
IHC-P Use at an assay dependent concentration. For paraffin embedded tissue a TUF pretreatment is recommended.
WB Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 相关性Cytokeratin is a family of basic and acidic proteins, present in dermal tissues. Each cytokeratin is formed by heterotetramers of different types of keratin, that span from 1 to 18. In keratinized epidermis, 50 kD keratin is present in the basal layer, while 56.5 kD keratin is present in suprabasal layers, where as 58 kD keratin is present in the basal and suprabasal layers, while 65 to 67 kD keratin is present in the cells above the basal layers.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • CK1 antibody
    • Cytokeratin 1 antibody
    • EHK1 antibody
    • K1 antibody
    • Keratin 1 antibody
    • KRT1 antibody
    • KRT1A antibody
    • KRTA antibody
    see all

Anti-pan Keratin antibody [80] 图像

  • ab8068 staining human foreskin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in 10mM Citrate buffer pH 6.0 in boiling water bath for 10 minutes, followed by cooling at room temperature for 30 minutes. The blocking was done using 1%BSA/5% normal donkey serum in PBS pH 7.4 for 2 hours at room temperature. The primary antibody, diluted 1/10 (PBS pH 7.4, 1%BSA, 0.1% sodium azide) and incubated with sample for 16 hours at 4°C. A HRP-conjugated donkey polyclonal to mouse Ig, diluted 1/1000 was used as the secondary.

    See Abreview

  • ICC/IF image of ab8068 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-pan Keratin antibody [80] (ab8068) at 1/250 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 49 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Overlay histogram showing A431 cells stained with ab8068 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8068, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab8068 staining pan Keratin in rat glioblastoma cell line C6 by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab8068 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a Cy3 conjugated goat anti-mouse polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    See Abreview

Anti-pan Keratin antibody [80] (ab8068)参考文献

This product has been referenced in:
  • Kuo S  et al. Biochemical indicators of implantation success of tissue-engineered oral mucosa. J Dent Res 94:78-84 (2015). Read more (PubMed: 25348542) »
  • O'Brien K  et al. Identification of the critical therapeutic entity in secreted Hsp90a that promotes wound healing in newly re-standardized healthy and diabetic pig models. PLoS One 9:e113956 (2014). Read more (PubMed: 25464502) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line)
Specification human glioblastoma cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100 in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (glioblastoma cell line C6)
Specification glioblastoma cell line C6
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100 in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 26 2012

Thank you for contacting Abcam. I have looked through our catalogue to see if we have any prepared slides of human callus or keratinocytes. I have not been able to find anything that matches your requirements, but I have placed a link below that will s...

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Thank you for your question. We do not have any information about cross reactivity with pig. Please do not hesitate to contact us in case of any additional questions or remarks.

Thank you for getting back to me and for explaining a little bit more about your experiments. It may be worth applying antibodies which recognize pig albumin or serum (ab79960, ab50337, ab79957). Unfortunately, currently we do not have many anti...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. As our Abpromise indicates, in the event that a prod...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (foreskin)
Specification foreskin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate pH 6
Permeabilization No
Blocking step 1%BSA plus 5% normal donkey serum in PBS pH 7.4 as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 30 2008

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (epithelial)
Specification epithelial
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1%
Username

Dr. todd french

Verified customer

提交于 Feb 20 2007

Thank you for your enquiry. When conducting IHC, it is not advisable to use antibodies which were raised in the same species as the target sample e.g. a mouse mAb against mouse sample. The secondary Ab may recognise endogenous IgG in your sample tis...

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Unfortunately, we are not specialists in this area and we do not want to recommend a product that is unsuitable for your needs. We suggest that you refer to the latest literature in this field.

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