Anti-pan-Hexokinase抗体[4D7] (ab58979)

概述

  • 产品名称Anti-pan-Hexokinase抗体[4D7]
    参阅全部 pan-Hexokinase 一抗
  • 描述
    小鼠单克隆抗体[4D7] to pan-Hexokinase
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-917 of Human pan-Hexokinase

  • 阳性对照
    • HeLa or Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab58979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000.
ELISA Use at an assay dependent dilution.
ICC/IF 1/1000.

靶标

  • 相关性Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.
  • 细胞定位Mitochondrion outer membrane.
  • 数据库链接
  • 别名
    • Brain form hexokinase antibody
    • Hexokinase 1 antibody
    • Hexokinase type I antibody
    • HK I antibody
    • HK1 ta antibody
    • HK1 tb antibody
    • HK1 tc antibody
    • HKI antibody
    • HXK1 antibody
    see all

Anti-pan-Hexokinase antibody [4D7] 图像

  • All lanes : Anti-pan-Hexokinase antibody [4D7] (ab58979) at 1/1000 dilution

    Lane 1 : Hexokinase 1 recombinant protein at 0.02 µg
    Lane 2 : Hexokinase 2 recombinant protein at 0.02 µg
    Lane 3 : Hexokinase 3 recombinant protein at 0.02 µg
    Lane 4 : Hexokinase 4 (Glucokinase) recombinant protein at 0.02 µg
    Lane 5 : Hexokinase 1 recombinant protein at 0.1 µg
    Lane 6 : Hexokinase 2 recombinant protein at 0.1 µg
    Lane 7 : Hexokinase 3 recombinant protein at 0.1 µg
    Lane 8 : Hexokinase 4 (Glucokinase) recombinant protein at 0.1 µg

    Secondary
    HRP-Conjugated Goat Anti-Mouse
    Developed using the ECL technique
  • All lanes : Anti-pan-Hexokinase antibody [4D7] (ab58979) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Mouse cerebellum lysate

    Secondary
    HRP-conjugated Goat anti-Mouse antibody.
    Developed using the ECL technique

    Observed band size : 52,100 kDa (why is the actual band size different from the predicted?)
  • ab58979 at 1/1000 dilution, staining Hexokinase in HeLa cells by Immunohistochemistry/Immunofluorescence. An Alexa Fluor® 488-conjugated antibody was used as the secondary antibody, and Hoechst 33342 was used as the counterstain in Blue.

Anti-pan-Hexokinase antibody [4D7] (ab58979)参考文献

ab58979 has not yet been referenced specifically in any publications.

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