Anti-pan Cytokeratin抗体[C-11] (Phycoerythrin) (ab52460)

概述

  • 产品名称Anti-pan Cytokeratin抗体[C-11] (Phycoerythrin)
    参阅全部 pan Cytokeratin 一抗
  • 描述
    小鼠单克隆抗体[C-11] to pan Cytokeratin (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性ab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    Reacts with all species (recognized epitope conserved within all species)
  • 免疫原

    Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Prior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
  • 克隆单克隆
  • 克隆编号C-11
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1-2µl for 106 cells.

(in a 100µl cell suspension).



ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • 细胞定位Cytoplasmic

Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin) 图像

  • Intracellular flow cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using ab52460.

    Recommended conc. 0.5 μg/ml

    Overlay with Isotype mouse IgG1 control.

Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin) (ab52460)参考文献

This product has been referenced in:
  • Rajendran S  et al. Preclinical evaluation of gene delivery methods for the treatment of loco-regional disease in breast cancer. Exp Biol Med (Maywood) 236:423-34 (2011). Read more (PubMed: 21444371) »
  • Rajendran S  et al. Targeting of breast metastases using a viral gene vector with tumour-selective transcription. Anticancer Res 31:1627-35 (2011). Read more (PubMed: 21617219) »

See all 3 Publications for this product

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We have a PE- conjugated antibody against cytokeratin, catalogue item ab52460:

Click here (or use the following: http://www.abcam.com/index.html?datasheet=52460).

For collagen I, we have onl...

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