Anti-pan Cytokeratin抗体[C-11] (ab118817)

概述

  • 产品名称Anti-pan Cytokeratin抗体[C-11]
    参阅全部 pan Cytokeratin 一抗
  • 描述
    小鼠单克隆抗体[C-11] to pan Cytokeratin
  • 特异性Reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.
  • 经测试应用适用于: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: all Mammals
  • 免疫原

    Tissue, cells or virus corresponding to Human pan Cytokeratin.

  • 阳性对照
    • Human Breast tissue.

性能

应用

Our Abpromise guarantee covers the use of ab118817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • 细胞定位Cytoplasmic
  • 数据库链接

Anti-pan Cytokeratin antibody [C-11] 图像

  • ab118817, at 10 µg/ml, staining Keratin in formalin-fixed, paraffin-embedded Human Breast tissue by Immunohistochemistry using a biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-pan Cytokeratin antibody [C-11] (ab118817)参考文献

ab118817 has not yet been referenced specifically in any publications.

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