Anti-pan Cytokeratin抗体[AE1/AE3] - BSA and Azide free (ab80826)

概述

  • 产品名称Anti-pan Cytokeratin抗体[AE1/AE3] - BSA and Azide free
    参阅全部 pan Cytokeratin 一抗
  • 描述
    小鼠单克隆抗体[AE1/AE3] to pan Cytokeratin - BSA and Azide free
  • 特异性Clone AE1 binds to the keratins of the acidic subfamily (56.5, 50, 48 and 40kDa). Clone AE3 binds to the keratins of the basic subfamily (65-67, 64, 59, 58, 56, and 52kDa).
  • 经测试应用适用于: Flow Cyt, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Cow, Human, Monkey
  • 免疫原

    Human epidermal keratin

  • 阳性对照
    • Skin. Lung carcinoma. IF: HepG2 cell line

性能

应用

Our Abpromise guarantee covers the use of ab80826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Boiling tissue sections in 1mM EDTA (pH 8.0), for 10-20min followed by cooling at RT for 20min is required.

靶标

  • 相关性Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • 细胞定位Cytoplasmic
  • 数据库链接

Anti-pan Cytokeratin antibody [AE1/AE3] - BSA and Azide free 图像

  • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human skin tissue using 1/50 ab80826, a peroxidase-conjugated secondary antibody and an AEC chromogen. Note cytoplasmic staining of epithelial cells.
  • ICC/IF image of ab80826 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80826, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Overlay histogram showing A431 cells stained with ab80826 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80826, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-pan Cytokeratin antibody [AE1/AE3] - BSA and Azide free (ab80826)参考文献

This product has been referenced in:
  • Polettini J  et al. Telomere Fragment Induced Amnion Cell Senescence: A Contributor to Parturition? PLoS One 10:e0137188 (2015). Read more (PubMed: 26397719) »
  • Woodcock-Mitchell J  et al. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol 95:580-8 (1982). IHC-P, ICC/IF ; Human . Read more (PubMed: 6183275) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer
Sample Rat Tissue sections (bladder)
Specification bladder
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Formaldehyde
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提交于 Sep 26 2014

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