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Our Abpromise guarantee covers the use of ab27988 in the following tested applications.
|IHC-P||1/10 - 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product requires protein digestion pretreatment (e.g. trypsin or pronase), or antigen retrieval using heat treatment prior to staining of paraffin sections.
|IHC-Fr||1/10 - 1/40.|
ab27988 staining Cytokeratin in Mouse tissue sections (Human tumor xenograft (Breast carcinoma)) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with MOM kit from Vector Laboratories for 1 hour at 15°C; antigen retrieval was by enzymatic in proteinase K. Samples were incubated with primary antibody (1/20) for 30 minutes at 15°C. A Biotin-conjugated Goat anti-mouse polyclonal (1/10) was used as the secondary antibody.