Anti-pan Cytokeratin抗体[AE1/AE3 + 5D3] (ab86734)

概述

  • 产品名称
    Anti-pan Cytokeratin抗体[AE1/AE3 + 5D3]
    参阅全部 pan Cytokeratin 一抗
  • 描述
    小鼠单克隆抗体[AE1/AE3 + 5D3] to pan Cytokeratin
  • 经测试应用
    适用于: IHC-P, Flow Cyt, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Goat, Chicken, Cat, Dog, Human, Pig
  • 免疫原

    Full length protein corresponding to pan Cytokeratin.

  • 阳性对照
    • Skin or adenocarcinoma This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

性能

应用

Our Abpromise guarantee covers the use of ab86734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Digest with Trypsin enzyme for 5 minutes at 37°C- or -for 15 minutes at RT.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.
IHC-Fr 1/200.

靶标

  • 相关性
    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • 细胞定位
    Cytoplasmic
  • 数据库链接

Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] 图像

  • Paraffin embedded, 0.1% Triton X-100 permeabilized mouse skin stained for pan Cytokeratin with ab86734 at a 1/100 dilution. Heat mediated - Buffer/Enzyme Used: Trypsin enzyme for 15 minutes at RT. 10% serum used to block for 1 hour at RT. Primary incubation for 16 hours at 4°C. Secondary: Goat polyclonal conjugated to biotin used at a 1/100 dilution.

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  • Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized human colon tissue stained for pan Cytokeratin with ab86734 (green) at a 1/200 dilution in immunohistological analysis of frozen sections. 5% BSA blocked for 2 hours at RT. Primary incubation time 12 hours at 4°C. Secondary mouse polyclonal conjugated to AlexaFluor® 488 used at a 1/200 dilution. Histone H3K27me3 was stained with red color.

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  • ab86734 staining pan Cytokeratin in pig small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/250 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

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  • ab86734 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86734 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) HeLa, Hek293. HepG2, and MCF-7 cells, also in formaldehyde fixed (4%, 10min) HeLa, Hek293, and MCF-7 cells at 1/100 dilution.

  • ab86734 staining pan Cytokeratin in rat squamous epithelial tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed using Proteinase K. Samples were then blocked using 1% BSA for 25 minutes at 25°C, followed by incubation with ab86734 at a 1/100 dilution for 16 hours at 25°C. The secondary used was an undiluted HRP goat polyclonal.

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  • Formaldehyde fixed dog lung tissue stained for pan Cytokeratin with ab86734 at a 1/250 dilution. Heat mediated - Buffer/Enzyme Used: Citric acid. 1% BSA used for blocking for 10 minutes at RT. Primary incubation for 2 hours at RT in TBS/BSA/Azide. Secondary: Goat polyclonal conjugated to biotin used at a 1/200 dilution.

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  • Formaldehyde fixed goat lung tissue stained for pan Cytokeratin with ab86734 at a 1/250 dilution. Heat mediated - Buffer/Enzyme Used: Citric acid. 1% BSA used for blocking for 10 minutes at RT. Primary incubation for 2 hours at RT in TBS/BSA/Azide. Secondary: Goat polyclonal conjugated to biotin used at a 1/200 dilution.

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  • Formaldehyde fixed chicken lung tissue stained for pan Cytokeratin with ab86734 at a 1/200 dilution. Heat mediated - Buffer/Enzyme Used: Citric acid. 1% BSA used for blocking for 10 minutes at RT. Primary incubation for 16 hours at RT in TBS/BSA/Azide. Secondary: Goat polyclonal conjugated to biotin used at a 1/200 dilution.

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  • Skin stained for pan Cytokeratin with ab86734 at a 1/100 dilution.

  • ab8673 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab8673 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.

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  • Overlay histogram showing A431 cells stained with ab86734 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86734, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)参考文献

This product has been referenced in:
  • Yumoto K  et al. Axl is required for TGF-ß2-induced dormancy of prostate cancer cells in the bone marrow. Sci Rep 6:36520 (2016). Human . Read more (PubMed: 27819283) »
  • Seidman JD  et al. Lipid cell (steroid cell) tumor of the ovary: immunophenotype with analysis of potential pitfall due to endogenous biotin-like activity. Int J Gynecol Pathol 14:331-8 (1995). Read more (PubMed: 8598336) »

See all 5 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Colon tissue)
Permeabilization
Yes - 0.1%triton x-100 in PBS
Specification
Colon tissue
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 06 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Trypsin enzyme for 15 minutes at RT
Permeabilization
Yes - 0.1% Triton x-100
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 16 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Pig Tissue sections (Small intestine)
Specification
Small intestine
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Sep 11 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Goat Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Jan 13 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Chicken Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Jan 13 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Cat Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Jan 13 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Bladder)
Specification
Bladder
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Jan 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Lung)
Specification
Lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Mar 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Specification
Skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Mar 01 2012

Thank you for contacting us. The antibody ab86734 is a cocktail of three clones. The IgG concentrations of each clone for the current GR53946-1 are as follows: clone 5D3 is at 130µg/mL clone AE1 is at 25µg/mL clone AE3 is at 46.3µg/...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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