Anti-pan Cytokeratin抗体[5D3 & LP34], prediluted (ab848)


  • 产品名称Anti-pan Cytokeratin抗体[5D3 & LP34], prediluted
    参阅全部 pan Cytokeratin 一抗
  • 描述
    小鼠单克隆抗体[5D3 & LP34] to pan Cytokeratin, prediluted
  • 特异性This antibody is a cocktail of two different clones; 5D3 and LP53. This antibody stains cytokeratin 5,6,8 and 18. This antibody has been designed for a broad spectrum screening of cytokeratins. It stains carcinomas of simple and squamous origin.
  • 经测试应用适用于: IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Cytokeratins from human breast carcinoma cell line MCF-7 and detergent insoluble fraction of psoriatic epidermis.

  • 常规说明Isotype IgG2a and IgG1



Our Abpromise guarantee covers the use of ab848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
    Proteolytic digestion of tissue sections is required prior to immunostaining.
  • 靶标

    • 相关性Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
    • 细胞定位Cytoplasmic

    Anti-pan Cytokeratin antibody [5D3 & LP34], prediluted (ab848)参考文献

    ab848 has not yet been referenced specifically in any publications.

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