Anti-pan CEACAM抗体[TET2] (ab26283)

概述

  • 产品名称Anti-pan CEACAM抗体[TET2]
    参阅全部 pan CEACAM 一抗
  • 描述
    小鼠单克隆抗体[TET2] to pan CEACAM
  • 特异性ab26283 is a broadly reactive antibody which reacts with most of the CEACAM molecules characterized to date (except CEACAM3, CEACAM4 and CEACAM7)4. It can be used to detect CEACAM1 (BGP/CD66a), CEACAM5 (CEA/CD66e), CEACAM6 (NCA/CD66c) and CEACAM8 (CGM6/CD66b) on transiently transfected BOSC23 cells by flow cytometry.
  • 经测试应用适用于: Flow Cyt, ELISA, WB, IHC-Fr, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    ab26283 was generated by immunisation of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors.

性能

应用

Our Abpromise guarantee covers the use of ab26283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 21645414NK cells were isolated from whole PBMC by negative magnetic bead fractionation.

靶标

  • 相关性CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
  • 细胞定位Cell Membrane
  • 数据库链接
  • 别名
    • Antigen CD66 antibody
    • Biliary glycoprotein antibody
    • Carcinoembryonic antigen related cell adhesion molecule 1 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 3 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 4 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 5 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 6 antibody
    • CD66a antigen antibody
    • CD66D antibody
    • CD66e antibody
    • CEACAM1 antibody
    • CEACAM3 antibody
    • CEACAM4 antibody
    • CEACAM5 antibody
    • CEACAM6 antibody
    see all

Anti-pan CEACAM antibody [TET2] 图像

  • Overlay histogram showing Caco 2 cells stained with ab26283 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26283, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-pan CEACAM antibody [TET2] (ab26283)参考文献

This product has been referenced in:
  • Moreland AJ  et al. Characterization of killer immunoglobulin-like receptor genetics and comprehensive genotyping by pyrosequencing in rhesus macaques. BMC Genomics 12:295 (2011). IP ; Rhesus monkey . Read more (PubMed: 21645414) »
  • Graham SA  et al. Identification of Neutrophil Granule Glycoproteins as Lewisx-containing Ligands Cleared by the Scavenger Receptor C-type Lectin. J Biol Chem 286:24336-49 (2011). WB . Read more (PubMed: 21561871) »

See all 2 Publications for this product

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Merci de nous avoir contactés.

L'anti-pan CEACAM [TET2] (ab26283) est un anticorps purifié sur protéine G et n'est pas conjugué à un fluorophore ou à une enzyme.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"