Anti-pan CEACAM抗体[D14HD11] (ab4567)

概述

  • 产品名称Anti-pan CEACAM抗体[D14HD11]
    参阅全部 pan CEACAM 一抗
  • 描述
    小鼠单克隆抗体[D14HD11] to pan CEACAM
  • 特异性Broadly reactive with high affinity (affinity to solid phase bound CEACAM 5: 2 x 1010 L/Mol [Jantscheff P et al.]) with most of the CEACAM molecules (except CEACAM 7 and CEACAM 8) transiently expressed on the cell surface of transfected BOSC23 cells. This antibody can be used to detect CEACAM 1 (BGP/CD66a), CEACAM 3 (CGM1/CD66d), CEACAM 4 (CGM 7), CEACAM 5 and CEACAM 6 (NCA/CD66c) on transfectants by flow cytometry.
  • 经测试应用适用于: Flow Cyt, ELISA, IHC-Fr, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native Carcino Embryonic Antigen (partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Jantscheff P et al.).

  • 常规说明Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

性能

应用

Our Abpromise guarantee covers the use of ab4567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/40.
WB 1/40.

靶标

  • 相关性CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
  • 细胞定位Cell Membrane
  • 数据库链接
  • 别名
    • Antigen CD66 antibody
    • Biliary glycoprotein antibody
    • Carcinoembryonic antigen related cell adhesion molecule 1 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 3 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 4 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 5 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 6 antibody
    • CD66a antigen antibody
    • CD66D antibody
    • CD66e antibody
    • CEACAM1 antibody
    • CEACAM3 antibody
    • CEACAM4 antibody
    • CEACAM5 antibody
    • CEACAM6 antibody
    see all

Anti-pan CEACAM antibody [D14HD11] (ab4567)参考文献

This product has been referenced in:
  • Johswich KO  et al. In vivo adaptation and persistence of Neisseria meningitidis within the nasopharyngeal mucosa. PLoS Pathog 9:e1003509 (2013). IF ; Mouse . Read more (PubMed: 23935487) »
  • Patel PC  et al. Inside-out signaling promotes dynamic changes in the carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1) oligomeric state to control its cell adhesion properties. J Biol Chem 288:29654-69 (2013). WB ; Human . Read more (PubMed: 24005674) »

See all 3 Publications for this product

Product Wall

Thank you for your inquiry. I was liaising with the laboratories to find out more about our pan-CEACAM- and CEACAM1-antibodies. Unfortunately, it turned out that we do not have any suitable antibody available: The immunogens of our CEACAM1 anti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"