概述

  • 产品名称
    Anti-pan Cadherin抗体
    参阅全部 pan Cadherin 一抗
  • 描述
    兔多克隆抗体to pan Cadherin
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, ICC, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Zebrafish
    预测可用于: Chicken, Cow, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human pan Cadherin.

    (Peptide available as ab17098.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; 3T3.

      This antibody gave a positive signal in the following tissue lysates: Mouse Heart Normal; Mouse Muscle normal; Human Heart Normal; Rat Heart Normal.

      This antibody gave a positive signal in the following cell lines: HeLa.

      This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Human Liver Normal.

性能

应用

Our Abpromise guarantee covers the use of ab16505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 2 µg/ml.

A diffuse signal is seen throughout the cells if higher concentrations are used (5-10µg/ml). We have had reports that the antibody works less well in this application in murine (3T3) cells. 

ICC Use a concentration of 2 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 100 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

图片

  • All lanes : Anti-pan Cadherin antibody (ab16505) at 1 µg/ml

    Lane 1 : Human heart lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 100 kDa
    Observed band size: 125-140 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 40 kDa, 65 kDa, 75 kDa, 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • ICC/IF image of ab16505 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16505, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • IHC image of pan Cadherin staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16505, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-pan Cadherin antibody (ab16505) at 1 µg/ml

    Lane 1 : Mouse heart
    Lane 2 : HeLa cell lysate
    Lane 3 : 3T3 cell lysate
    Lane 4 : Mouse muscle
    Lane 5 : Human heart
    Lane 6 : Mouse heart with Human pan Cadherin peptide (ab17098) at 1 µg/ml
    Lane 7 : HeLa cell lysate with Human pan Cadherin peptide (ab17098) at 1 µg/ml
    Lane 8 : 3T3 cell lysate with Human pan Cadherin peptide (ab17098) at 1 µg/ml
    Lane 9 : Mouse muscle with Human pan Cadherin peptide (ab17098) at 1 µg/ml
    Lane 10 : Human heart with Human pan Cadherin peptide (ab17098) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit conjugated to Alexafluor 680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 100 kDa

  • HeLa cells fixed in methanol and stained with ab16505 (2µg/ml). The cells were fixed in 100% methanol for 6 minutes at -20°C, then washed once in PBS. The 2 images show the cells stained with different secondary antibodies, Donkey anti Rabbit FITC (image A) and Donkey anti Rabbit Cy3 (image B). In each case ab16505 stains the plasma membrane.  In image A ab16505 is stained green and in image B ab16505 is stained red. In both images the DNA is stained with DAPI (blue).

  • Immunofluorescence analysis of HeLa cells, staining pan Cadherin (red) with ab16505.

    Cells were fixed with paraformaldehyde, permeabilized in methanol and blocked for 1 hour at room temperature in DPBS containing 2 mg/mL BSA, 1% fish gelatin, 0.1% Triton-X 100 and 5% goat serum. Cells were then incubated for 1 hour at room temperature with the primary antibody diluted in blocking buffer. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

文献

This product has been referenced in:
  • Haas J  et al. Genomic structural variations lead to dysregulation of important coding and non-coding RNA species in dilated cardiomyopathy. EMBO Mol Med 10:107-120 (2018). WB ; Human . Read more (PubMed: 29138229) »
  • Hirth S  et al. Paxillin and Focal Adhesion Kinase (FAK) Regulate Cardiac Contractility in the Zebrafish Heart. PLoS One 11:e0150323 (2016). WB . Read more (PubMed: 26954676) »

See all 40 Publications for this product

客户评价及客户问答

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human ovarian cystedenocarcinoma tissues)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: bicarbonate buffer pH 6.0
Permeabilization
No
Specification
human ovarian cystedenocarcinoma tissues
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 11 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Human Cell (ovarian carcinoma SKOV-3 cell line)
Specification
ovarian carcinoma SKOV-3 cell line
Permeabilization
Yes - 0.1% triton X for 5 min at RT
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 26 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (Day 9.5 embryo)
Specification
Day 9.5 embryo
Permeabilization
Yes - 0.1% saponin
Fixative
Paraformaldehyde
Username

Dr. Nikhil Mittal

Verified customer

提交于 Sep 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Specification
HEK293
Fixative
100% Ethanol
Permeabilization
No
Blocking step
(agent) for 30 minute(s) · Concentration: 0.1% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jan 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (kidney)
Specification
kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 May 21 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (ALL cell lines plasma membrane prep)
Loading amount
10 µg
Specification
ALL cell lines plasma membrane prep
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in PBS-
Username

Dr. Mark Holland

Verified customer

提交于 Mar 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Differentiated PC12 cells)
Specification
Differentiated PC12 cells
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Oct 12 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
40 µg
Specification
brain
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Oct 10 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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