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Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human pan Cadherin.
(Peptide available as ab17098.)
Our Abpromise guarantee covers the use of ab16505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2 µg/ml.
A diffuse signal is seen throughout the cells if higher concentrations are used (5-10µg/ml). We have had reports that the antibody works less well in this application in murine (3T3) cells.
|ICC||Use a concentration of 2 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 100 kDa).|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ICC/IF image of ab16505 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16505, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
HeLa cells fixed in methanol and stained with ab16505 (2µg/ml). The cells were fixed in 100% methanol for 6 minutes at -20°C, then washed once in PBS. The 2 images show the cells stained with different secondary antibodies, Donkey anti Rabbit FITC (image A) and Donkey anti Rabbit Cy3 (image B). In each case ab16505 stains the plasma membrane. In image A ab16505 is stained green and in image B ab16505 is stained red. In both images the DNA is stained with DAPI (blue).