Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-15% GRADIENT GEL)
Sample
Human Cell lysate - whole cell (OVARIAN CANCER CELL LINES)
Specification
OVARIAN CANCER CELL LINES
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: GOAT ANTI-MOUSE IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: ~49 kDa
Exposure
10 second(s)
Additional data
Additional Notes
University of Oklahoma, Health Science Centre
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 30 2014