Application
Western blot
Sample
Human Cell lysate - other (HeLa, U2OS: Purified mitochondria lysate)
Loading amount
2e+006 cells
Specification
HeLa, U2OS: Purified mitochondria lysate
Gel Running Conditions
Reduced Denaturing (8% polyacrylamide gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Dako goat anti mouse HRP conjugated
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL plus
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 45 kDa
Positive control
Purified mitochondrial extract
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提交于 Aug 09 2012