Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (foreskin)
Specification
foreskin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate pH 6
Permeabilization
No
Blocking step
1%BSA plus 5% normal donkey serum in PBS pH 7.4 as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/10
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS pH 7.4, 1%BSA, ), 0.1% sodium azide
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Additional data
Additional Notes
Peroxidase block in 60:40:1 PBS:MetOH: 30% H2O2 10'. Antigen retrieval: Boiling water bath for 20 min, followed by cooling at RT for 30 min. Lower Ab concentration would very likely be satisfactory but we haven't done that optimization yet.
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提交于 Sep 30 2008