Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Colon tissue)
Permeabilization
Yes - 0.1%triton x-100 in PBS
Specification
Colon tissue
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Alexa Flour 488
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
We did IF in human colon tissue. Cytokeratin was stained with green color, and histone H3K27me3 was stained with red color.
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提交于 May 06 2016