Application
Western blot
Sample
Human Cell lysate - other (ALL cell lines plasma membrane prep)
Loading amount
10 µg
Specification
ALL cell lines plasma membrane prep
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in PBS-
Other product details
Dilution
1/5000
Incubation time
12 hour(s) and 0 minute(s)
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~120-110 kDa Non-specific: ~80 kDa
Positive control
Whole cell extracts of cell lines used
Additional data
Additional Notes
I used this antibody to compare plasma membrane and non-membrane extracts. The plasma membrane is positive for cadherin but so where the non-membrane fractions. Not the best antibody as a cell marker for plasma membrane in my view if you wish to compare "non-membrane" fractions to it for analysis of membrane purity. A good antibody but you definately need other markers as well to analyse plasma membrane purity.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Mark Holland
Verified customer
提交于 Mar 05 2007