RabMAb

Anti-PAM抗体[EPR2643(2)] (ab109175)

概述

  • 产品名称Anti-PAM抗体[EPR2643(2)]
    参阅全部 PAM 一抗
  • 描述
    兔单克隆抗体[EPR2643(2)] to PAM
  • 经测试应用适用于: WBmore details
    不适用于: ICC/IF or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PAM (C terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: Human fetal heart tissue lysate and PC-3 cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab109175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
  • 应用说明Is unsuitable for ICC/IF or IHC-P.
  • 靶标

    • 功能Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
    • 序列相似性In the C-terminal section; belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.
      In the N-terminal section; belongs to the copper type II ascorbate-dependent monooxygenase family.
      Contains 5 NHL repeats.
    • 细胞定位Membrane and Secreted. Secreted from secretory granules.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AMD_HUMAN antibody
      • PAL antibody
      • PAM antibody
      • Pancreatic peptidylglycine alpha amidating monooxygenase antibody
      • Peptidyl alpha amidating enzyme antibody
      • Peptidyl alpha hydroxyglycine alpha amidating lyase antibody
      • Peptidyl-alpha-hydroxyglycine alpha-amidating lyase antibody
      • Peptidylamidoglycolate lyase antibody
      • Peptidylglycine 2 hydroxylase antibody
      • Peptidylglycine alpha amidating monooxygenase antibody
      • Peptidylglycine alpha hydroxylating monooxygenase antibody
      • PHM antibody
      see all

    Anti-PAM antibody [EPR2643(2)] 图像

    • Anti-PAM antibody [EPR2643(2)] (ab109175) at 1/10000 dilution (purified) + Human fetal heart tissue lysate at 20 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 108 kDa
      Observed band size : 108 kDa
      Blocking buffer: 5% NFDM/TBST.
      Dilution buffer: 1%BSA/TBST.
    • All lanes : Anti-PAM antibody [EPR2643(2)] (ab109175) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal heart tissue lysate
      Lane 2 : PC-3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 108 kDa

    Anti-PAM antibody [EPR2643(2)] (ab109175)参考文献

    ab109175 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"