概述

  • 产品名称Anti-PAM抗体
    参阅全部 PAM 一抗
  • 描述
    山羊多克隆抗体to PAM
  • 特异性This antibody is expected to recognise all reported isoforms (NP_000910.2; NP_620121.1; NP_620176.1 and NP_620177.1).
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    DQEKEDDGSESEE

    , corresponding to C terminal amino acids 947-959 of Human PAM (NP_000910.2; NP_620121.1; NP_620176.1; NP_620177.1)

  • 阳性对照
    • Human kidney lysates.

性能

应用

Our Abpromise guarantee covers the use of ab77592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明Peptide ELISA: Antibody detection limit dilution 1/4000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
    • 序列相似性In the C-terminal section; belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.
      In the N-terminal section; belongs to the copper type II ascorbate-dependent monooxygenase family.
      Contains 5 NHL repeats.
    • 细胞定位Membrane and Secreted. Secreted from secretory granules.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AMD_HUMAN antibody
      • PAL antibody
      • PAM antibody
      • Pancreatic peptidylglycine alpha amidating monooxygenase antibody
      • Peptidyl alpha amidating enzyme antibody
      • Peptidyl alpha hydroxyglycine alpha amidating lyase antibody
      • Peptidyl-alpha-hydroxyglycine alpha-amidating lyase antibody
      • Peptidylamidoglycolate lyase antibody
      • Peptidylglycine 2 hydroxylase antibody
      • Peptidylglycine alpha amidating monooxygenase antibody
      • Peptidylglycine alpha hydroxylating monooxygenase antibody
      • PHM antibody
      see all

    Anti-PAM antibody 图像

    Anti-PAM antibody (ab77592)参考文献

    ab77592 has not yet been referenced specifically in any publications.

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